An Acidic Exopolysaccharide from Haloarcula hispanica ATCC33960 and Two Genes Responsible for Its Synthesis
Joint Authors
Lü, Yang
Lu, Hua
Wang, Shiwei
Han, Jing
Xiang, Hua
Jin, Cheng
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A 1.1 × 106 Da acidic exopolysaccharide (EPS) was purified from an extremely halophilic archaeon Haloarcula hispanica ATCC33960 with a production of 30 mg L−1 when grown in AS-168 medium, which mainly composed of mannose and galactose with a small amount of glucose in a molar ratio of 55.9 : 43.2 : 0.9.
Two glycosyltransferase genes (HAH_1662 and HAH_1667) were identified to be responsible for synthesis of the acidic EPS.
Deletion of either HAH_1662 or HAH_1667 led to loss of the acidic EPS.
The mutants displayed a different cell surface morphology, retarded growth in low salty environment, an increased adhesion, and swimming ability.
Our results suggest that biosynthesis of the acidic EPS might act as an adaptable mechanism to protect the cells against harsh environments.
American Psychological Association (APA)
Lü, Yang& Lu, Hua& Wang, Shiwei& Han, Jing& Xiang, Hua& Jin, Cheng. 2017. An Acidic Exopolysaccharide from Haloarcula hispanica ATCC33960 and Two Genes Responsible for Its Synthesis. Archaea،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1125463
Modern Language Association (MLA)
Lü, Yang…[et al.]. An Acidic Exopolysaccharide from Haloarcula hispanica ATCC33960 and Two Genes Responsible for Its Synthesis. Archaea No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1125463
American Medical Association (AMA)
Lü, Yang& Lu, Hua& Wang, Shiwei& Han, Jing& Xiang, Hua& Jin, Cheng. An Acidic Exopolysaccharide from Haloarcula hispanica ATCC33960 and Two Genes Responsible for Its Synthesis. Archaea. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1125463
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125463