A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens
Joint Authors
Wang, Liangliang
Lupa, Boguslaw
Whitman, William B.
Long, Feng
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Many hydrogenotrophic methanogens use either H2 or formate as the major electron donor to reduce CO2 for methane production.
The conventional cultivation of these organisms uses H2 and CO2 as the substrate with frequent replenishment of gas during growth.
H2 is explosive and requires an expensive gassing system to handle safely.
Formate is as an ideal alternative substrate from the standpoints of both economy and safety but leads to large changes in the culture pH during growth.
Here, we report that glycylglycine is an inexpensive and nontoxic buffer suitable for growth of Methanococcus maripaludis and Methanothermococcus okinawensis.
This cultivation system is suitable for growth on liquid as well as solid medium in serum bottles.
Moreover, it allows cultivation of liter scale cultures without expensive fermentation equipment.
This formate cultivation system provides an inexpensive and flexible alternative for the growth of formate-utilizing, hydrogenotrophic methanogens.
American Psychological Association (APA)
Long, Feng& Wang, Liangliang& Lupa, Boguslaw& Whitman, William B.. 2017. A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens. Archaea،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1125467
Modern Language Association (MLA)
Long, Feng…[et al.]. A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens. Archaea No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1125467
American Medical Association (AMA)
Long, Feng& Wang, Liangliang& Lupa, Boguslaw& Whitman, William B.. A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens. Archaea. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1125467
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125467