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Biodegradation of Atrazine by the Novel Klebsiella variicola Strain FH-1
Joint Authors
Zhang, Jinpeng
Liang, Shuang
Wang, Xinhong
Lu, Zhongbin
Sun, Peng
Zhang, Hao
Sun, Feng-Jie
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Bacterial strain FH-1 with high efficiency of degrading Atrazine is separated by means of enrichment culture from the soil applied with Atrazine for many years.
FH-1, recognized as Klebsiella variicola based on phylogenetic analysis of 16S rDNA sequences, can grow with Atrazine which is the sole nitrogen source.
In fluid inorganic salt medium, the optimal degradation temperature, pH value, and initial concentration of Atrazine are 25°C, 9.0, and 50 mg L–1, respectively, and the degradation rate of Atrazine by strain FH-1 reached 81.5% in 11 d of culture.
The degrading process conforms to the kinetics equation of pesticide degradation.
Among the metal ions tested, Zn2+ (0.2 mM) has the most significant effect of facilitation on the degradation of Atrazine.
In the fluid medium with Zn2+, the degradation rate of Atrazine is increased to 72.5%, while the Cu2+ (0.2 mM) inhibits the degradation of Atrazine.
The degradation products of Atrazine by strain FH-1 were identified as HEIT (2-hydroxyl-4-ethylamino-6-isopropylamino-1,3,5-triazine), MEET (2-hydroxyl-4,6-bis(ethylamino)-1,3,5-triazine), and AEEO (4,6-bis(ethylamino)-1,3,5-triazin-2(1H)-one) by HPLC-MS/MS.
Three genes (atzC, trzN, and trzD) encoding for Atrazine degrading enzymes were identified by PCR and sequencing in strain FH-1.
This study provides additional theoretical support for the application of strain FH-1 in bioremediation of fields polluted by Atrazine.
American Psychological Association (APA)
Zhang, Jinpeng& Liang, Shuang& Wang, Xinhong& Lu, Zhongbin& Sun, Peng& Zhang, Hao…[et al.]. 2019. Biodegradation of Atrazine by the Novel Klebsiella variicola Strain FH-1. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125574
Modern Language Association (MLA)
Zhang, Jinpeng…[et al.]. Biodegradation of Atrazine by the Novel Klebsiella variicola Strain FH-1. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1125574
American Medical Association (AMA)
Zhang, Jinpeng& Liang, Shuang& Wang, Xinhong& Lu, Zhongbin& Sun, Peng& Zhang, Hao…[et al.]. Biodegradation of Atrazine by the Novel Klebsiella variicola Strain FH-1. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125574
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125574