AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alphaNF-kappaBVEGF Pathway
Joint Authors
Yu, Xiaoyu
Wang, Yan
Qiu, Huilei
Song, Hongtao
Feng, Di
Jiang, Yang
Deng, Shuzhe
Meng, Hongxue
Geng, Jingshu
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
Ovarian carcinoma represents one of the deadliest malignancies among female cancer patients.
Astrocyte-elevated gene-1 (AEG-1) participates in the ontogenesis of multiple human malignant diseases.
Here we evaluated AEG-1, hypoxia-inducible factor- (HIF-) 1α, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and vascular endothelial growth factor (VEGF) amounts in hypoxia induced ovarian carcinoma cells.
This study aimed to explore the mechanism by which AEG-1 regulates metastasis in hypoxia induced ovarian carcinoma.
Patients and Methods.
AEG-1, HIF-1α, and VEGF protein amounts were evaluated by immunohistochemistry in 40 and 170 normal ovary and ovarian cancer tissue specimens, respectively.
In addition, AEG-1, HIF-1α, NF-κB, and VEGF mRNA and protein levels were determined by reverse quantified RT-PCR and WB, respectively, at different time periods (0–24 h) in epithelial ovarian cancer (EOC) SKOV3 cells treated in a hypoxia incubator.
Furthermore, NF-κB and VEGF gene and protein expression levels in AEG-1 knockdown EOC cells were quantitated by RT-PCR and WB, respectively.
Results.
AEG-1, HIF-1α, and VEGF amounts were significantly elevated in EOC tissue samples compared with normal ovary specimens (p<0.001).
Positive expression of HIF-1α and AEG-1 was associated with higher metastatic rate (p<0.01), lower FIGO stage (p<0.001), and degree of differentiation (p<0.001).
Meanwhile, EOC SKOV3 cells grew upon exposure to hypoxia for 8 h (p<0.001); at this time point, AEG-1, HIF-1α, NF-κB, and VEGF amounts peaked (p<0.001), at both the gene and the protein levels.
After AEG-1 knockdown, HIF-1α, NF-κB, and VEGF amounts were significantly decreased in EOC SKOV3 cells, also under hypoxic conditions (p<0.01).
Conclusions.
As an independent prognostic factor, AEG-1 was found to be significantly associated with hypoxia in ovarian cancer by regulating the HIF-1alpha/NF-kappaB/VEGF pathway.
Therefore, AEG-1 may be useful in determining disease stage and prognosis in ovarian cancer.
American Psychological Association (APA)
Yu, Xiaoyu& Wang, Yan& Qiu, Huilei& Song, Hongtao& Feng, Di& Jiang, Yang…[et al.]. 2018. AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alphaNF-kappaBVEGF Pathway. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125670
Modern Language Association (MLA)
Yu, Xiaoyu…[et al.]. AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alphaNF-kappaBVEGF Pathway. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1125670
American Medical Association (AMA)
Yu, Xiaoyu& Wang, Yan& Qiu, Huilei& Song, Hongtao& Feng, Di& Jiang, Yang…[et al.]. AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alphaNF-kappaBVEGF Pathway. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125670
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125670