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Potential Regulatory Effects of miR-182-3p in Osteosarcoma via Targeting EBF2
Joint Authors
Yang, Qiwei
Wang, Qingyu
Zhang, Guizhen
Chen, Gaoyang
Li, Zhaoyan
Yu, Wenqing
Du, Zhenwu
Song, Yang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Osteosarcoma (OS) is one of the most common primary malignant bone tumors in adolescents with a high mortality rate.
MicroRNA (miRNA) is a kind of noncoding RNAs and has been proved to participate in many physiological processes.
Many miRNAs have been reported to act as function regulators in OS.
In our study, the miRNA and gene expression profiles of OS were downloaded from GEO Datasets and the differential expression analysis was performed using GEO2R.
58 up- and 126 downregulated miRNAs were found.
In the three OS gene profiles, 125 up- and 27 downregulated genes were found to be differentially expressed in at least two profiles.
The miRNA-mRNA networks were constructed to predict the potential target genes of 10 most up- and downregulated miRNA.
Venn analysis was used to detect the coexpressed differentially expressed genes (DEGs).
EBF2, one of the upregulated DEGs, was also a potential target gene of miR-182-3p.
Knockdown and overexpression of miR-182-3p resulted in overexpression and downexpression of EBF2 separately.
Luciferase reporter gene experiment further verified the binding site of miR-182-3p and EBF2.
CCK8 assay showed that miR-182-3p knockdown can further enhance the proliferation activity of OS cells, while overexpressing miR-182-3p can inhibit the proliferation activity of OS cells.
Our research indicated that downexpression of miR-182-3p in OS cells results in overexpression of EBF2 and promotes the progression of OS.
American Psychological Association (APA)
Chen, Gaoyang& Yu, Wenqing& Li, Zhaoyan& Wang, Qingyu& Yang, Qiwei& Du, Zhenwu…[et al.]. 2019. Potential Regulatory Effects of miR-182-3p in Osteosarcoma via Targeting EBF2. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1125682
Modern Language Association (MLA)
Chen, Gaoyang…[et al.]. Potential Regulatory Effects of miR-182-3p in Osteosarcoma via Targeting EBF2. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1125682
American Medical Association (AMA)
Chen, Gaoyang& Yu, Wenqing& Li, Zhaoyan& Wang, Qingyu& Yang, Qiwei& Du, Zhenwu…[et al.]. Potential Regulatory Effects of miR-182-3p in Osteosarcoma via Targeting EBF2. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1125682
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125682