Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells
Joint Authors
Adhikari, A.
Thabrew, M. Ira
Tennekoon, Kamani H.
Samarakoon, Sameera R.
Sivakumaran, Nivethika
Ediriweera, Meran K.
Malavige, Neelika
Shrestha, Ram Lal (Swagat)
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Current breast cancer therapies have limitations in terms of increased drug resistance resulting in short-term efficacy, thus demanding the discovery of new therapeutic agents.
In this study, cytotoxic activity and apoptotic effects of govaniadine isolated from Corydalis govaniana Wall.
roots were determined on human breast cancer (MCF-7) cells.
The SRB assay result revealed that govaniadine led to dose- and time-dependent cytotoxic effect in MCF-7 cells along with less cytotoxicity against MCF-10A cells.
Govaniadine-induced apoptosis was also accompanied by upregulation of Bax, p53, and Survivin mRNA expression as assessed by real time PCR analysis.
Flow cytometric analysis with Annexin V and PI staining indicated that govaniadine is a potent inducer of apoptosis in MCF-7 cell lines.
Distinctive morphological changes contributed to apoptosis and DNA laddering were observed in govaniadine-treated MCF-7 cells.
Caspase-7 was significantly activated in treated MCF-7 cells.
Govaniadine-treated MCF-7 cells also showed enhanced levels of intracellular reactive oxygen species (ROS) and glutathione S-transferase (GST) and decreased levels of glutathione (GSH).
The results indicate that govaniadine has potent and selective cytotoxic effects against MCF-7 cells and the potential to induce caspase 7 dependent apoptosis in MCF-7 cells by activation of pathways that lead to oxidative stress.
American Psychological Association (APA)
Sivakumaran, Nivethika& Samarakoon, Sameera R.& Adhikari, A.& Ediriweera, Meran K.& Tennekoon, Kamani H.& Malavige, Neelika…[et al.]. 2018. Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125692
Modern Language Association (MLA)
Sivakumaran, Nivethika…[et al.]. Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1125692
American Medical Association (AMA)
Sivakumaran, Nivethika& Samarakoon, Sameera R.& Adhikari, A.& Ediriweera, Meran K.& Tennekoon, Kamani H.& Malavige, Neelika…[et al.]. Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125692
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125692