Neuronal Proteomic Analysis of the Ubiquitinated Substrates of the Disease-Linked E3 Ligases Parkin and Ube3a
Joint Authors
Martinez, Aitor
Ramirez, Juanma
Osinalde, Nerea
Arizmendi, Jesus M.
Mayor, Ugo
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-06
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Both Parkin and UBE3A are E3 ubiquitin ligases whose mutations result in severe brain dysfunction.
Several of their substrates have been identified using cell culture models in combination with proteasome inhibitors, but not in more physiological settings.
We recently developed the Ubbio strategy to isolate ubiquitinated proteins in flies and have now identified by mass spectrometry analysis the neuronal proteins differentially ubiquitinated by those ligases.
This is an example of how flies can be used to provide biological material in order to reveal steady state substrates of disease causing genes.
Collectively our results provide new leads to the possible physiological functions of the activity of those two disease causing E3 ligases.
Particularly, in the case of Parkin the novelty of our data originates from the experimental setup, which is not overtly biased by acute mitochondrial depolarisation.
In the case of UBE3A, it is the first time that a nonbiased screen for its neuronal substrates has been reported.
American Psychological Association (APA)
Martinez, Aitor& Ramirez, Juanma& Osinalde, Nerea& Arizmendi, Jesus M.& Mayor, Ugo. 2018. Neuronal Proteomic Analysis of the Ubiquitinated Substrates of the Disease-Linked E3 Ligases Parkin and Ube3a. BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125694
Modern Language Association (MLA)
Martinez, Aitor…[et al.]. Neuronal Proteomic Analysis of the Ubiquitinated Substrates of the Disease-Linked E3 Ligases Parkin and Ube3a. BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1125694
American Medical Association (AMA)
Martinez, Aitor& Ramirez, Juanma& Osinalde, Nerea& Arizmendi, Jesus M.& Mayor, Ugo. Neuronal Proteomic Analysis of the Ubiquitinated Substrates of the Disease-Linked E3 Ligases Parkin and Ube3a. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125694
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125694