Suppression of IL-6 Gene by shRNA Augments Gemcitabine Chemosensitization in Pancreatic Adenocarcinoma Cells

Abstract EN

Pancreatic adenocarcinoma has an exceedingly poor prognosis, accounting for five-year survival of less than 5%.

Presently, improving the efficacy of pancreatic adenocarcinoma treatment has been the focus of medical researchers worldwide.

Recently, it has been suggested that deregulation of interleukin- (IL-) 6 is caused by a key gene involved in the beginning and development of pancreatic adenocarcinoma.

Herein, we investigated whether suppression of IL-6 could augment gemcitabine sensitivity in the PANC-1 cells.

We found considerably higher expression of IL-6 in pancreatic adenocarcinoma tissues than that in the adjacent nontumorous tissues.

Suppression of IL-6 by shRNA resulted in apoptosis as well as inhibition of cell proliferation and tumorigenicity.

In addition, suppression of IL-6 remarkably promoted antitumor effect of gemcitabine, indicating that the combination of shRNA targeting IL-6 with gemcitabine may provide a potential clinical approach for pancreatic cancer therapy.