Targeting Immune-Related Molecules in Cancer Therapy: A Comprehensive In Vitro Analysis on Patient-Derived Tumor Models
Joint Authors
Klar, Ernst
Scheinpflug, Philine
Witt, Anika
Maletzki, Claudia
Linnebacher, Michael
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This study investigated the impact of immune-related pathway inhibition, among them indolamine 2,3-dioxygenase (IDO), alone and together with immune cells on growth and viability of colorectal cancer (CRC) cells.
A panel of patient-derived CRC cell lines with different molecular characteristics (CpG island methylator phenotype, chromosomal, and microsatellite instability) was included.
Initial phenotyping of CRC cell lines (n=17) revealed high abundance of immunosuppressive checkpoint-molecules in general, but an individual profile for IDO.
Presence of immune-related molecules was independent of the molecular subtype.
Selective treatment of CRC cell lines showing high or low IDO expression (n=2 cell lines each) was performed with single agents and combinations of Indoximod, Curcumin, and Gemcitabine with and without the addition of peripheral blood lymphocytes (PBL) in an allogeneic setting.
All substances affected CRC cell growth in a cell line specific manner.
The combination of Curcumin and Gemcitabine proved to be most effective in tumor cell elimination.
Functional read-out analyses identified cellular senescence, after both single and combined treatment.
Curcumin alone exerted strong cytotoxic effects by inducing early and late apoptosis.
Necrosis was not detectable at all.
Addition of lymphocytes generally boosted antitumoral effects of all IDO-inhibitors, with up to 80 % cytotoxicity for the Curcumin treatment.
Here, no obvious differences became apparent between individual cell lines.
Combined application of Curcumin and low-dose chemotherapy is a promising strategy to kill tumor target cells and to stimulate antitumoral immune responses.
American Psychological Association (APA)
Maletzki, Claudia& Scheinpflug, Philine& Witt, Anika& Klar, Ernst& Linnebacher, Michael. 2019. Targeting Immune-Related Molecules in Cancer Therapy: A Comprehensive In Vitro Analysis on Patient-Derived Tumor Models. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125722
Modern Language Association (MLA)
Maletzki, Claudia…[et al.]. Targeting Immune-Related Molecules in Cancer Therapy: A Comprehensive In Vitro Analysis on Patient-Derived Tumor Models. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1125722
American Medical Association (AMA)
Maletzki, Claudia& Scheinpflug, Philine& Witt, Anika& Klar, Ernst& Linnebacher, Michael. Targeting Immune-Related Molecules in Cancer Therapy: A Comprehensive In Vitro Analysis on Patient-Derived Tumor Models. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125722