Induction and Characterization of Tetraploids from Seeds of Bletilla striata (Thunb.) Reichb.f.
Joint Authors
Li, Meiya
Jiang, Fu-Sheng
Ding, Bin
Ding, Zhi-Shan
Huang, Weipeng
Pan, Jieli
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Bletilla striata (Thunb.), an ornamental and medicinal plant, is on the list of endangered plants in China.
Its pseudobulb is abundant in polysaccharide and has been used for centuries as a herbal remedy.
However, a recent rise in demand has placed it at risk of extinction, and therefore, research on its propagation and genetic improvement is essential.
Since polyploids tend to possess advantageous qualities, we incubated B.
striata seeds with colchicine with the aim of creating tetraploid plantlets.
Aseptic seeds treated with 0.1% colchicine for 7 days showed the highest tetraploid induction rate of 40.67 ± 0.89%.
Compared with the wild-type, the tetraploids could be identified by their morphological characteristics including larger stomata at a lower density, larger leaf blades, and a thicker petiole.
Contents of polysaccharide and phenolic compounds were also determined in the tetraploid pseudobulbs, revealing significantly higher values than in the wild-type.
In vitro colchicine treatment can therefore be used to successfully produce B.
striata tetraploids with superior pseudobulbs.
American Psychological Association (APA)
Li, Meiya& Ding, Bin& Huang, Weipeng& Pan, Jieli& Ding, Zhi-Shan& Jiang, Fu-Sheng. 2018. Induction and Characterization of Tetraploids from Seeds of Bletilla striata (Thunb.) Reichb.f.. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125731
Modern Language Association (MLA)
Li, Meiya…[et al.]. Induction and Characterization of Tetraploids from Seeds of Bletilla striata (Thunb.) Reichb.f.. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1125731
American Medical Association (AMA)
Li, Meiya& Ding, Bin& Huang, Weipeng& Pan, Jieli& Ding, Zhi-Shan& Jiang, Fu-Sheng. Induction and Characterization of Tetraploids from Seeds of Bletilla striata (Thunb.) Reichb.f.. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1125731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125731