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High Glucose Enhances the OdontoOsteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway
Joint Authors
Yu, Jinhua
Wang, Yanping
Wang, Yanqiu
Lu, Yadie
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
The transport and metabolism of glucose are important during mammalian development.
High glucose can mediate the biological characteristics of mesenchymal stem cells (MSCs).
However, the role of high glucose in the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) is unclear.
Materials and Methods.
SCAPs were isolated and identified in vitro.
Then, SCAPs were cultured in normal α-MEM and high glucose α-MEM separately.
MTT assay was applied to observe the proliferation of SCAPs.
ALP activity, alizarin red staining, real-time RT-PCR, and western blot were used to detect the odonto/osteogenic capacity of SCAPs as well as the participation of NF-κB pathway.
Results.
SCAPs in 25mmol/L glucose group expressed the maximum proteins of RUNX2 and ALP as compared with those in 5, 10, and 15 mmol/L groups.
MTT assay showed that 25 mmol/L glucose suppressed the proliferation of SCAPs.
ALP assay, alizarin red staining, real-time RT-PCR, and western blot showed 25 mmol/L high glucose can obviously enhance the odonto/osteogenic capacity of SCAPs.
Moreover, the NF-κB pathway was activated in 25mmol/L glucose-treated SCAPs and the odonto/osteogenic differentiation was inhibited following the inhibition of NF-κB signaling pathway.
Conclusions.
High glucose can enhance the odonto/osteogenic capacity of SCAPs via NF-κB pathway.
American Psychological Association (APA)
Wang, Yanping& Wang, Yanqiu& Lu, Yadie& Yu, Jinhua. 2019. High Glucose Enhances the OdontoOsteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1125788
Modern Language Association (MLA)
Wang, Yanping…[et al.]. High Glucose Enhances the OdontoOsteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1125788
American Medical Association (AMA)
Wang, Yanping& Wang, Yanqiu& Lu, Yadie& Yu, Jinhua. High Glucose Enhances the OdontoOsteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1125788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125788