Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System
Joint Authors
Yang, Zhao-Yang
Li, Xiao-Guang
Li, Manli
Zhao, Wen
Gao, Yudan
Hao, Peng
Shang, Junkui
Duan, Hongmei
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-27
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Bone marrow mesenchymal stem cells undergo differentiation to different lineages with different efficiencies when induced by different factors.
We added a bFGF-chitosan controlled release system (bFGF-CCRS) as an inducer into conditioned medium to facilitate the oriented differentiation of BMSCs into neural lineage cells (eventually mature neurons); furthermore, we synchronized BMSCs to the G0/G1 phase via serum starvation to observe the effect of the inducer on the differentiation direction and efficiency.
The nonsynchronized group, chitosan alone (not loaded with bFGF) group, soluble bFGF group, and conditioned medium group served as controls, and we observed the dynamic process of differentiation of BMSCs into neural lineage cells at different time points after the beginning of coculture.
We analyzed the binding patterns of bFGF and chitosan and assayed the expression differences of key factors (FGFR1, ERK, and c-fos) and molecular switches (BTG2) that regulate the transformation from cell proliferation to differentiation.
We also investigated the potential molecular mechanism of BMSC differentiation into neural lineage cells at a high percentage when induced by bFGF-CCRS.
American Psychological Association (APA)
Li, Manli& Zhao, Wen& Gao, Yudan& Hao, Peng& Shang, Junkui& Duan, Hongmei…[et al.]. 2019. Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System. BioMed Research International،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1125806
Modern Language Association (MLA)
Li, Manli…[et al.]. Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System. BioMed Research International No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1125806
American Medical Association (AMA)
Li, Manli& Zhao, Wen& Gao, Yudan& Hao, Peng& Shang, Junkui& Duan, Hongmei…[et al.]. Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1125806
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125806