Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory
Joint Authors
Liao, Duan-Fang
Lin, Li-mei
Zhao, Ling-jia
Deng, Jing
Xiong, Su-hui
Tang, Jie
Li, Ya-mei
Xia, Bo-hou
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection.
In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield.
The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions.
The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC).
PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC).
Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities.
Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells.
These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.
American Psychological Association (APA)
Lin, Li-mei& Zhao, Ling-jia& Deng, Jing& Xiong, Su-hui& Tang, Jie& Li, Ya-mei…[et al.]. 2018. Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1125845
Modern Language Association (MLA)
Lin, Li-mei…[et al.]. Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1125845
American Medical Association (AMA)
Lin, Li-mei& Zhao, Ling-jia& Deng, Jing& Xiong, Su-hui& Tang, Jie& Li, Ya-mei…[et al.]. Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1125845
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125845