Keap1 Cystenine 151 as a Potential Target for Artemisitene-Induced Nrf2 Activation
Joint Authors
Liu, Shanshan
Xu, Shengmei
Wei, Renrong
Cui, Zhizhong
Wu, Xiaoyun
Wei, Renxiong
Xie, Li
Zhou, Yingye
Li, Wenjuan
Chen, Weimin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Artemisitene (ATT) activates the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) by increasing its stabilization and reducing ubiquitination.
The cysteine (Cys) residues of the cytosolic Nrf2 repressor Kelch-like ECH-associated protein-1 (Keap1) function as redox sensors and may be crucial in activating Nrf2.
To determine whether ATT-induced Nrf2 activation is dependent on the modification of Keap1 and to elucidate the underlying mechanism, we transfected cell lines with six different Keap1 mutant constructs, each with a Cys (−77, −151, −257, −273, −288, and −297) to Ser substitution.
Only the Cys151Ser mutant prevented ATT-mediated activation of Nrf2, indicating that the Cys151 residue of Keap1 likely interacts with ATT and is essential for Nrf2 stabilization and transcription of downstream genes.
Our finding provides a pharmacological basis for using artemisitene against oxidative stress-related diseases.
American Psychological Association (APA)
Liu, Shanshan& Xu, Shengmei& Wei, Renrong& Cui, Zhizhong& Wu, Xiaoyun& Wei, Renxiong…[et al.]. 2019. Keap1 Cystenine 151 as a Potential Target for Artemisitene-Induced Nrf2 Activation. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125881
Modern Language Association (MLA)
Liu, Shanshan…[et al.]. Keap1 Cystenine 151 as a Potential Target for Artemisitene-Induced Nrf2 Activation. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1125881
American Medical Association (AMA)
Liu, Shanshan& Xu, Shengmei& Wei, Renrong& Cui, Zhizhong& Wu, Xiaoyun& Wei, Renxiong…[et al.]. Keap1 Cystenine 151 as a Potential Target for Artemisitene-Induced Nrf2 Activation. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125881
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125881