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Mitochondrial Coenzyme Q Protects Sepsis-Induced Acute Lung Injury by Activating PI3KAktGSK-3βmTOR Pathway in Rats
Joint Authors
Li, Ruirui
Ren, Tao
Zeng, Jianqiong
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of our study was to assess the effects of mitochondrial coenzyme Q (MitoQ) on sepsis-induced acute lung injury (ALI) and investigate its possible mechanisms.
The cecal ligation and puncture (CLP) method was used to establish a septic ALI model.
Rats were randomly divided into Con group, CLP group, MitoQ group, and MitoQ + LY294002 group.
The survival rate of the rats was recorded, and the survival rate curve was plotted.
Moreover, the ratio of wet/dry weight (W/D) in lung tissue was measured.
The activity of myeloperoxidase (MPO) was measured by using the MPO colorimetric activity assay kit.
The levels of high-mobility group box 1 (HMGB1) and interleukin-6 (IL-6), macrophage inflammatory protein 2 (MIP2), and keratinocyte chemoattractant (KC) were analyzed by ELISA.
The histopathological changes were measured by HE staining, and the lung injury was scored.
TUNEL assay was applied to detect the apoptotic cells in lung tissue.
The protein expressions were detected by western blot.
MitoQ increased the survival rate and alleviated pulmonary edema in septic ALI rats.
In addition, MitoQ inhibited the MPO activity and decreased the levels of HMGB1 and IL-6.
After treatment with MitoQ, alveolar wall edema, inflammatory cell infiltration, and red blood cell exudation were relieved.
MitoQ inhibited cell apoptosis in lung tissue of septic ALI rats.
Meanwhile, MitoQ treatment remarkedly increased the expression of p-Akt, p-GSK-3β, and p-mTOR but decreased Bax, caspase-3, caspase-9, Beclin-1, and LC-3II/LC-3I.
The effects of MitoQ were significantly reversed by the PI3K inhibitor (LY294002).
Our study demonstrated that MitoQ could protect sepsis-induced acute lung injury by activating the PI3K/Akt/GSK-3β/mTOR pathway in rats.
American Psychological Association (APA)
Li, Ruirui& Ren, Tao& Zeng, Jianqiong. 2019. Mitochondrial Coenzyme Q Protects Sepsis-Induced Acute Lung Injury by Activating PI3KAktGSK-3βmTOR Pathway in Rats. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1125911
Modern Language Association (MLA)
Li, Ruirui…[et al.]. Mitochondrial Coenzyme Q Protects Sepsis-Induced Acute Lung Injury by Activating PI3KAktGSK-3βmTOR Pathway in Rats. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1125911
American Medical Association (AMA)
Li, Ruirui& Ren, Tao& Zeng, Jianqiong. Mitochondrial Coenzyme Q Protects Sepsis-Induced Acute Lung Injury by Activating PI3KAktGSK-3βmTOR Pathway in Rats. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1125911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125911