Identification of Hub Genes and Key Pathways Associated with Two Subtypes of Diffuse Large B-Cell Lymphoma Based on Gene Expression Profiling via Integrated Bioinformatics
Joint Authors
Liu, Zijian
Meng, Jingshu
Li, Xiaoqian
Zhu, Fang
Liu, Tao
Wu, Gang
Zhang, Liling
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-24
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
There is a significant difference in prognosis between the germinal center B-cell (GCB) and activated B-cell (ABC) subtypes of diffuse large B-cell lymphoma (DLBCL).
However, the signaling pathways and driver genes involved in these disparate subtypes are ambiguous.
This study integrated three cohort profile datasets, including 250 GCB samples and 250 ABC samples, to elucidate potential candidate hub genes and key pathways involved in these two subtypes.
Differentially expressed genes (DEGs) were identified.
After Gene Ontology functional enrichment analysis of the DEGs, protein-protein interaction (PPI) network and sub-PPI network analyses were conducted using the STRING database and Cytoscape software.
Subsequently, the Oncomine database and the cBioportal online tool were employed to verify the alterations and differential expression of the 8 hub genes (MME, CD44, IRF4, STAT3, IL2RA, ETV6, CCND2, and CFLAR).
Gene set enrichment analysis was also employed to identify the intersection of the key pathways (JAK-STAT, FOXO, and NF-κB pathways) validated in the above analyses.
These hub genes and key pathways could improve our understanding of the process of tumorigenesis and the underlying molecular events and may be therapeutic targets for the precise treatment of these two subtypes with different prognoses.
American Psychological Association (APA)
Liu, Zijian& Meng, Jingshu& Li, Xiaoqian& Zhu, Fang& Liu, Tao& Wu, Gang…[et al.]. 2018. Identification of Hub Genes and Key Pathways Associated with Two Subtypes of Diffuse Large B-Cell Lymphoma Based on Gene Expression Profiling via Integrated Bioinformatics. BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125925
Modern Language Association (MLA)
Liu, Zijian…[et al.]. Identification of Hub Genes and Key Pathways Associated with Two Subtypes of Diffuse Large B-Cell Lymphoma Based on Gene Expression Profiling via Integrated Bioinformatics. BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1125925
American Medical Association (AMA)
Liu, Zijian& Meng, Jingshu& Li, Xiaoqian& Zhu, Fang& Liu, Tao& Wu, Gang…[et al.]. Identification of Hub Genes and Key Pathways Associated with Two Subtypes of Diffuse Large B-Cell Lymphoma Based on Gene Expression Profiling via Integrated Bioinformatics. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1125925
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125925