The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in dbdb Mice through miR-192miR-200b and PTENAKT and ZEB2ECM Pathways
Joint Authors
Yu, Shunjie
Zhao, Haoran
Yang, Wenjing
Amat, Ramila
Peng, Jun
Li, Yike
Deng, Kai
Mao, Xinmin
Jiao, Yi
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The study aims to investigate the effects of the alcohol extract of Coreopsis tinctoria Nutt (AC) on diabetic nephropathy (DN) mice.
A total of 30 db/db (DN) mice were divided into 3 groups, which were treated with AC (300 mg/kg/day), metformin (180 mg/kg/day), or saline by gavage for 10 weeks.
Ten db/m mice treated with saline were used as normal control (NC group).
Body weight (BW) and fasting blood glucose (FBG), HbA1c, 24 h urinary albumin excretion (UAE), and renal pathological fibrosis were analyzed.
Expression of miR-192, miR-200b, and proteins in the PTEN/PI3K/AKT pathway was analyzed by qPCR or western blot.
The DN mice had significantly higher BW, FBG, and 24 h UAE, as well as more severe pathological fibrosis when compared with NC.
Treatment of AC could decrease BW, FBG, and 24 h UAE and alleviated kidney damage.
Compared with the NC group, expressions of miR-192 and miR-200b were increased, whereas their target proteins (ZEB2 and PTEN) were reduced in the kidneys of DN mice, which further modulated the expression of their downstream proteins PI3K p85α, P-AKT, P-smad3, and COL4 α1; these proteins were increased in the kidneys of DN mice.
In contrast, AC treatment reversed the expression changes of these proteins.
These findings demonstrate that AC may protect the kidneys of DN mice by decreasing miR-192 and miR-200b, which could further regulate their target gene expression and modulate the activity of the PTEN/PI3K/AKT pathway to reduce the degree of renal fibrosis.
American Psychological Association (APA)
Yu, Shunjie& Zhao, Haoran& Yang, Wenjing& Amat, Ramila& Peng, Jun& Li, Yike…[et al.]. 2019. The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in dbdb Mice through miR-192miR-200b and PTENAKT and ZEB2ECM Pathways. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125940
Modern Language Association (MLA)
Yu, Shunjie…[et al.]. The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in dbdb Mice through miR-192miR-200b and PTENAKT and ZEB2ECM Pathways. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1125940
American Medical Association (AMA)
Yu, Shunjie& Zhao, Haoran& Yang, Wenjing& Amat, Ramila& Peng, Jun& Li, Yike…[et al.]. The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in dbdb Mice through miR-192miR-200b and PTENAKT and ZEB2ECM Pathways. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125940
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125940