Peptide Mix from Olivancillaria hiatula Interferes with Cell-to-Cell Communication in Pseudomonas aeruginosa
Joint Authors
Gasu, Edward Ntim
Ahor, Hubert Senanu
Borquaye, Lawrence Sheringham
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Bacteria in biofilms are encased in an extracellular polymeric matrix that limits exposure of microbial cells to lethal doses of antimicrobial agents, leading to resistance.
In Pseudomonas aeruginosa, biofilm formation is regulated by cell-to-cell communication, called quorum sensing.
Quorum sensing facilitates a variety of bacterial physiological functions such as swarming motility and protease, pyoverdine, and pyocyanin productions.
Peptide mix from the marine mollusc, Olivancillaria hiatula, has been studied for its antibiofilm activity against Pseudomonas aeruginosa.
Microscopy and microtiter plate-based assays were used to evaluate biofilm inhibitory activities.
Effect of the peptide mix on quorum sensing-mediated processes was also evaluated.
Peptide mix proved to be a good antibiofilm agent, requiring less than 39 μg/mL to inhibit 50% biofilm formation.
Micrographs obtained confirmed biofilm inhibition at 1/2 MIC whereas 2.5 mg/mL was required to degrade preformed biofilm.
There was a marked attenuation in quorum sensing-mediated phenotypes as well.
At 1/2 MIC of peptide, the expression of pyocyanin, pyoverdine, and protease was inhibited by 60%, 72%, and 54%, respectively.
Additionally, swarming motility was repressed by peptide in a dose-dependent manner.
These results suggest that the peptide mix from Olivancillaria hiatula probably inhibits biofilm formation by interfering with cell-to-cell communication in Pseudomonas aeruginosa.
American Psychological Association (APA)
Gasu, Edward Ntim& Ahor, Hubert Senanu& Borquaye, Lawrence Sheringham. 2019. Peptide Mix from Olivancillaria hiatula Interferes with Cell-to-Cell Communication in Pseudomonas aeruginosa. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125956
Modern Language Association (MLA)
Gasu, Edward Ntim…[et al.]. Peptide Mix from Olivancillaria hiatula Interferes with Cell-to-Cell Communication in Pseudomonas aeruginosa. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1125956
American Medical Association (AMA)
Gasu, Edward Ntim& Ahor, Hubert Senanu& Borquaye, Lawrence Sheringham. Peptide Mix from Olivancillaria hiatula Interferes with Cell-to-Cell Communication in Pseudomonas aeruginosa. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1125956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125956