Role of PKR in the Inhibition of Proliferation and Translation by Polycystin-1
Joint Authors
Tang, Yan
Yang, JungWoo
Zheng, Wang
Tang, Jingfeng
Chen, Xing-Zhen
Yang, Jianzheng
Shi, Guang
Wang, Zuocheng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Autosomal dominant polycystic kidney disease (ADPKD) is mainly caused by mutations in the PKD1 (~85%) or PKD2 (~15%) gene which, respectively, encode polycystin-1 (PC1) and polycystin-2 (PC2).
How PC1 regulates cell proliferation and apoptosis has been studied for decades but the underlying mechanisms remain controversial.
Protein kinase RNA-activated (PKR) is activated by interferons or double-stranded RNAs, inhibits protein translation, and induces cell apoptosis.
In a previous study, we found that PC1 reduces apoptosis through suppressing the PKR/eIF2α signaling.
Whether and how PKR is involved in PC1-inhibited proliferation and protein synthesis remains unknown.
Here we found that knockdown of PKR abolishes PC1-inhibited proliferation and translation.
Because suppressed PKR-eIF2α signaling/activity by PC1 would stimulate, rather than inhibit, the proliferation and translation, we examined the effect of dominant negative PKR mutant K296R that has no kinase activity and found that it enhances the inhibition of proliferation and translation by PC1.
Thus, our study showed that inhibition of cell proliferation and protein synthesis by PC1 is mediated by the total expression but not the kinase activity of PKR, possibly through physical association.
American Psychological Association (APA)
Tang, Yan& Shi, Guang& Yang, JungWoo& Zheng, Wang& Tang, Jingfeng& Chen, Xing-Zhen…[et al.]. 2019. Role of PKR in the Inhibition of Proliferation and Translation by Polycystin-1. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125960
Modern Language Association (MLA)
Tang, Yan…[et al.]. Role of PKR in the Inhibition of Proliferation and Translation by Polycystin-1. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1125960
American Medical Association (AMA)
Tang, Yan& Shi, Guang& Yang, JungWoo& Zheng, Wang& Tang, Jingfeng& Chen, Xing-Zhen…[et al.]. Role of PKR in the Inhibition of Proliferation and Translation by Polycystin-1. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1125960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1125960