Long Noncoding RNA Expression Profile in BV2 Microglial Cells Exposed to Lipopolysaccharide
Joint Authors
Li, Yajuan
Li, Qingmin
Wang, Cunjuan
Li, Shengde
Yu, Lingzhi
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Neuropathic pain, which is one of the most common forms of chronic pain, seriously increases healthcare costs and impairs patients’ quality of life with an incidence of 7–10% worldwide.
Microglia cell activation plays a key role in the progression of neuropathic pain.
Better understanding of novel molecules modulating microglia cell activation and these underlying functions will extremely benefit the exploration of new treatment.
Recent studies suggested long noncoding RNAs may be involved in neuropathic pain.
However, its underlying functions and mechanisms in microglia cell activation remain unclear.
To identify the differentially expressed lncRNAs and predict their functions in the progression of microglia cell activation, GSE103156 was analyzed using integrated bioinformatics methods.
The expression levels of selected lncRNAs and mRNAs were determined by real-time PCR.
In the present study, a total of 56 lncRNAs and 298 mRNAs were significantly differentially expressed.
The differentially expressed mRNAs were mainly enriched in NF-kappa B signaling pathway, TNF signaling pathway, Toll-like receptor signaling pathway, and NOD-like receptor signaling pathway.
The top 10 hub genes were Tnf, Il6, Stat1, Cxcl10, Il1b, Tlr2, Irf1, Ccl2, Irf7, and Ccl5 in the PPI network.
Our results showed that Gm8989, Gm8979, and AV051173 may be involved in the progression of microglia cell activation.
Taken together, our findings suggest that lots of lncRNAs may be involved in BV2 microglia cell activation in vitro.
The findings may provide relevant information for the development of promising targets for the microglial cells activation of neuropathic pain in vivo in the future.
American Psychological Association (APA)
Li, Yajuan& Li, Qingmin& Wang, Cunjuan& Li, Shengde& Yu, Lingzhi. 2019. Long Noncoding RNA Expression Profile in BV2 Microglial Cells Exposed to Lipopolysaccharide. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1126002
Modern Language Association (MLA)
Li, Yajuan…[et al.]. Long Noncoding RNA Expression Profile in BV2 Microglial Cells Exposed to Lipopolysaccharide. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1126002
American Medical Association (AMA)
Li, Yajuan& Li, Qingmin& Wang, Cunjuan& Li, Shengde& Yu, Lingzhi. Long Noncoding RNA Expression Profile in BV2 Microglial Cells Exposed to Lipopolysaccharide. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1126002
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126002