Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells
Joint Authors
Peng, Jian
Wei, Hongkui
Zuo, Fangrui
Gu, Qiongyao
Li, Shengqing
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
As one of the essential amino acids, methionine (Met) plays an important role in biological events such as methylation and antioxidant properties besides its function in protein synthesis.
Different Met sources have been used in animal production, but their effects on Met metabolic pathways are not well understood.
In the present study, we investigated the effects of different Met sources (L-Met, DL-Met, DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA), and DL-methionyl-DL-methionine (DL-MM)) on the metabolism of Met in small intestinal porcine epithelial cell line (IPEC-J2) and the contents of extracellular Met sources.
The results showed that concentrations of intracellular Met, S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and the ratio of SAM to SAH in the DL-HMTBA group were significantly lower than that in other Met source groups, while the content of 5-methyltetrahydrofolate (5-MTHF) was significantly higher.
Moreover, the mRNA levels of MAT2A, AHcy, CBS, MTHFR, and MTR in the DL-HMTBA group were significantly higher than those in other Met source groups.
Further study showed that the total content of extracellular Met sources was highest in the DL-HMTBA group, followed by DL-MM group, followed by L-Met and DL-Met groups.
These results demonstrated that DL-HMTBA mainly affects the transmethylation and remethylation of Met and it can promote the trans-sulfur metabolism of Met when compared with other Met sources.
In addition, most DL-HMTBA and a small amount of DL-MM can escape the intestinal first-pass metabolism and then provide more extracellular Met sources than L-Met and DL-Met.
Therefore, this study can provide a theoretical basis for the selection of Met sources in livestock.
American Psychological Association (APA)
Zuo, Fangrui& Gu, Qiongyao& Li, Shengqing& Wei, Hongkui& Peng, Jian. 2019. Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1126057
Modern Language Association (MLA)
Zuo, Fangrui…[et al.]. Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1126057
American Medical Association (AMA)
Zuo, Fangrui& Gu, Qiongyao& Li, Shengqing& Wei, Hongkui& Peng, Jian. Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1126057
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126057