The Potential of Fluocinolone Acetonide to Mitigate Inflammation and Lipid Accumulation in 2D and 3D Foam Cell Cultures
Joint Authors
Venkatraman, Subbu
Ng, Kee Woei
Nguyen, Luong T. H.
Muktabar, Aristo
Tang, Jinkai
Wong, Yee Shan
Thaxton, Colby S.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Inflammation plays an important role in all stages of atherosclerosis development.
Therefore, the use of anti-inflammatory drugs could reduce the risk of major adverse cardiovascular events due to atherosclerosis.
Herein, we explored the capacity of fluocinolone acetonide (FA), a glucocorticoid (GC), in modulating foam cell formation and response.
Human THP-1 derived foam cells were produced using 100 μg/mL oxidized low-density lipoproteins (OxLDL) and fetal bovine serum (1 and 10%).
2D cultures of these cells were treated with FA (0.1, 1, 10, and 50 μg/mL) in comparison with dexamethasone (Dex).
Results showed that treatment with 0.1 and 1 μg/mL FA and Dex improved foam cell survival.
FA and Dex also inhibited inflammatory cytokine (CD14, M-CSF, MIP-3α, and TNF-α) secretion.
Notably, at the concentration of 1 μg/mL, both FA and Dex reduced cholesteryl ester accumulation.
Compared to Dex, FA was significantly better in reducing lipid accumulation at the therapeutic concentrations of 1 and 10 μg/mL.
In a novel 3D foam cell spheroid model, FA was shown to be more effective than Dex in diminishing lipid accumulation, at the concentration of 0.1 μg/mL.
Taken together, FA was demonstrated to be effective in preventing both lipid accumulation and inflammation in foam cells.
American Psychological Association (APA)
Nguyen, Luong T. H.& Muktabar, Aristo& Tang, Jinkai& Wong, Yee Shan& Thaxton, Colby S.& Venkatraman, Subbu…[et al.]. 2018. The Potential of Fluocinolone Acetonide to Mitigate Inflammation and Lipid Accumulation in 2D and 3D Foam Cell Cultures. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1126061
Modern Language Association (MLA)
Nguyen, Luong T. H.…[et al.]. The Potential of Fluocinolone Acetonide to Mitigate Inflammation and Lipid Accumulation in 2D and 3D Foam Cell Cultures. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1126061
American Medical Association (AMA)
Nguyen, Luong T. H.& Muktabar, Aristo& Tang, Jinkai& Wong, Yee Shan& Thaxton, Colby S.& Venkatraman, Subbu…[et al.]. The Potential of Fluocinolone Acetonide to Mitigate Inflammation and Lipid Accumulation in 2D and 3D Foam Cell Cultures. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1126061
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126061