Study on the Effect of Ginsenosides Rb on Blood of Tumor Mice

Abstract EN

Objective.

The blood of cancer patients is in a state of hypercoagulability, easily leading to thrombosis.

Anemia is also a complication of tumors.

Anemia and thrombosis affect the treatment of tumor patients.

Methods.

Ginsenosides Rb were extracted from the stems and leaves of American ginseng using water-saturated ethanol and ethyl acetate in silica gel column.

Tumor mice model was established by injecting H22 hepatocellular carcinoma cells into the axilla of mice.

Mice were randomly divided into 6 groups: normal control group, model control group, positive control group, low dose group (7 mg/kg), middle dose group (14 mg/kg), and high dose group (35 mg/kg).

After 18 days, the blood was obtained by picking the eyeball of mice.

The levels of red blood cells (RBC), hemoglobin (HGB), neutrophils/lymphocytes radio (NLR), platelets (PLT), platelet distribution width (PDW), fibrinogen (FIB), and D-Dimer (D-D) were measured and compared in each group of mice.

Results.

The content of obtained ginsenosides Rb reached 90.05%.

This extraction process was simple and reliable.

Middle dose of ginsenosides Rb could significantly increase RBC and HGB levels (P<0.05).

Moreover, ginsenosides Rb could significantly reduce NLR, PLT, PDW, FIB, and D-D (P<0.01).

Conclusion.

ginsenosides Rb could significantly improve anaemia and hypercoagulation of blood in cancer mice.

Ginsenosides Rb are a potential anticoagulant and antianemia drug in treating cancer.