Significance of the Differential Peptidome in Multidrug-Resistant Tuberculosis
Joint Authors
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Most multidrug-resistant tuberculosis (MDR-TB) patients fail to receive a timely diagnosis and treatment.
Therefore, we explored the differentially expressed peptides in MDR-TB compared with drug-susceptible tuberculosis (DS-TB) patients using LC-MS/MS and Ingenuity Pathway Analysis (IPA) to analyse the potential significance of these differentially expressed peptides.
A total of 301 peptides were differentially expressed between MDR-TB and DS-TB groups.
Of these, 24 and 16 peptides exhibited presented high (fold change ≥ 2.0, P < 0.05) and low (fold change ≤ −2.0, P < 0.05) levels in MDR-TB.
Significant canonical pathways included the prothrombin activation system, coagulation system, and complement system.
In the network of differentially expressed precursor proteins, lipopolysaccharide (LPS) regulates many precursor proteins, including four proteins correlated with organism survival.
These four important differentially expressed proteins are prothrombin (F2), complement receptor type 2 (CR2), collagen alpha-2(V) chain (COL5A2), and inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4).
After addition of CR2 peptide, IL-6 mRNA expression in THP-1 cells decreased significantly in dose- and time-dependent manners.
Cumulatively, our study proposes potential biomarkers for MDR-TB diagnosis and enables a better understanding of the pathogenesis of MDR-TB.
The functions of differentially expressed peptides, especially CR2, in MDR-TB require further investigation.
American Psychological Association (APA)
Yang, Yan& Wu, Jianqing. 2019. Significance of the Differential Peptidome in Multidrug-Resistant Tuberculosis. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1126139
Modern Language Association (MLA)
Yang, Yan& Wu, Jianqing. Significance of the Differential Peptidome in Multidrug-Resistant Tuberculosis. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1126139
American Medical Association (AMA)
Yang, Yan& Wu, Jianqing. Significance of the Differential Peptidome in Multidrug-Resistant Tuberculosis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1126139
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126139