L-Carnitine Reduces Oxidative Stress and Promotes Cells Differentiation and Bone Matrix Proteins Expression in Human Osteoblast-Like Cells
Joint Authors
Luzi, Livio
Montesano, Anna
Villa, Isabella
Spinello, Alice
Rubinacci, Alessandro
Terruzzi, Ileana
Senesi, Pamela
Ferraretto, Anita
Bottani, Michela
Vacante, Fernanda
Bolamperti, Simona
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Bone fragility and associated fracture risk are major problems in aging.
Oxidative stress and mitochondrial dysfunction play a key role in the development of bone fragility.
Mitochondrial dysfunction is closely associated with excessive production of reactive oxygen species (ROS).
L-Carnitine (L-C), a fundamental cofactor in lipid metabolism, has an important antioxidant property.
Several studies have shown how L-C enhances osteoblastic proliferation and activity.
In the current study, we investigated the potential effects of L-C on mitochondrial activity, ROS production, and gene expression involved in osteoblastic differentiation using osteoblast-like cells (hOBs) derived from elderly patients.
The effect of 5mM L-C treatment on mitochondrial activity and L-C antioxidant activity was studied by ROS production evaluation and cell-based antioxidant activity assay.
The possible effects of L-C on hOBs differentiation were assessed by analyzing gene and protein expression by Real Time PCR and western blotting, respectively.
L-C enhanced mitochondrial activity and improved antioxidant defense of hOBs.
Furthermore, L-C increased the phosphorylation of Ca2+/calmodulin-dependent protein kinase II.
Additionally, L-C induced the phosphorylation of ERK1/2 and AKT and the main kinases involved in osteoblastic differentiation and upregulated the expression of osteogenic related genes, RUNX2, osterix (OSX), bone sialoprotein (BSP), and osteopontin (OPN) as well as OPN protein synthesis, suggesting that L-C exerts a positive modulation of key osteogenic factors.
In conclusion, L-C supplementation could represent a possible adjuvant in the treatment of bone fragility, counteracting oxidative phenomena and promoting bone quality maintenance.
American Psychological Association (APA)
Terruzzi, Ileana& Montesano, Anna& Senesi, Pamela& Villa, Isabella& Ferraretto, Anita& Bottani, Michela…[et al.]. 2019. L-Carnitine Reduces Oxidative Stress and Promotes Cells Differentiation and Bone Matrix Proteins Expression in Human Osteoblast-Like Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1126153
Modern Language Association (MLA)
Terruzzi, Ileana…[et al.]. L-Carnitine Reduces Oxidative Stress and Promotes Cells Differentiation and Bone Matrix Proteins Expression in Human Osteoblast-Like Cells. BioMed Research International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1126153
American Medical Association (AMA)
Terruzzi, Ileana& Montesano, Anna& Senesi, Pamela& Villa, Isabella& Ferraretto, Anita& Bottani, Michela…[et al.]. L-Carnitine Reduces Oxidative Stress and Promotes Cells Differentiation and Bone Matrix Proteins Expression in Human Osteoblast-Like Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1126153
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126153