Immunosuppressive Phenotype of Esophagus Tumors Stroma
Joint Authors
Kovaleva, Olga V.
Gratchev, Alexei
Rashidova, Madina A.
Samoilova, Daria V.
Podlesnaya, Polina A.
Mochalnikova, Valeria V.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Tumor-associated macrophages (TAMs) and tumor-infiltrating lymphocytes (TILs) contribute significantly to the development of immunosuppressive properties of a tumor.
In this study, we performed immunohistochemical analysis of immune cells of esophageal tumors stroma.
Methods.
Paraffin-embedded tissue specimens from 48 esophageal squamous cell carcinoma (ESCC) patients were retrospectively collected for immunohistochemical analysis of stromal cells.
For staining of macrophages, CD68, CD163, CD206, PU.1, and iNOS were used.
For T cell detection, CD8, CD3, and FOXP3 were used.
Also, we performed staining for PD-L1 that can be expressed on TAMs and tumor cells.
Clinicopathological and survival data were collected and analyzed using the χ2 and Fisher exact tests, Kaplan–Meier curves, and the log-rank test.
The correlation analysis was performed with Spearman’s rank correlation coefficient.
Results.
We found that FOXP3 expression was associated with age (p=0.042) and iNOS expression was associated with the disease stage (p=0.044).
In addition, FOXP3 and CD163 appeared to be markers of good prognosis (HR=0.4420, p=0.0325, and HR=0.4447, p=0.0456, respectively).
Significant association between PU.1+ and CD68+ macrophages (r=0.833; p≤0.001) and between PU.1+ and CD163+ macrophages (r=0.500; p≤0.001) was established; positive association between PU.1 and CD206 expression was also observed (r=0.250; p=0.043).
Conclusions.
Large amounts of CD163+ macrophages and FOXP3+ Т cells appear to be markers of good prognosis of ESCC.
The number of PU.1+ macrophages strongly correlates with the number of CD68+ macrophages; therefore, usage of PU.1 as a potential macrophage marker can be recommended for esophageal tumors.
American Psychological Association (APA)
Kovaleva, Olga V.& Rashidova, Madina A.& Samoilova, Daria V.& Podlesnaya, Polina A.& Mochalnikova, Valeria V.& Gratchev, Alexei. 2020. Immunosuppressive Phenotype of Esophagus Tumors Stroma. Analytical Cellular Pathology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1126175
Modern Language Association (MLA)
Kovaleva, Olga V.…[et al.]. Immunosuppressive Phenotype of Esophagus Tumors Stroma. Analytical Cellular Pathology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1126175
American Medical Association (AMA)
Kovaleva, Olga V.& Rashidova, Madina A.& Samoilova, Daria V.& Podlesnaya, Polina A.& Mochalnikova, Valeria V.& Gratchev, Alexei. Immunosuppressive Phenotype of Esophagus Tumors Stroma. Analytical Cellular Pathology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1126175
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126175