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Characterization and Genome Analysis of a Phthalate Esters-Degrading Strain Sphingobium yanoikuyae SHJ
Joint Authors
Feng, Liang
Liu, Hui
Cheng, Dandan
Mao, Xumei
Wang, Yan
Wu, Zhen
Wu, Qiong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A bacterium capable of utilizing dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP), and diisobuthyl phthalate (DIBP) as the sole carbon and energy source was isolated from shallow aquifer sediments.
The strain was identified as Sphingobium yanoikuyae SHJ based on morphological characteristics, 16S rDNA gene phylogeny, and whole genome average nucleotide identity (ANI).
The degradation half-life of DBP with substrate concentration of 8.5 and 50.0 mg/L by strain SHJ was 99.7 and 101.4 hours, respectively.
The optimum degradation rate of DBP by SHJ was observed at 30°C and weak alkaline (pH 7.5).
Genome sequence of the strain SHJ showed a circular chromosome and additional two circular plasmids with whole genome size of 5,669,383 bp and GC content of 64.23%.
Functional annotation of SHJ revealed a total of 5,402 genes, with 5,183 protein-encoding genes, 143 pseudogenes, and 76 noncoding RNA genes.
Based on genome annotation, 44 genes were identified to be involved in PAEs hydrolysis potentially.
Besides, a region with size of about 6.9 kb comprised of seven ORFs, which is located on the smaller plasmid pSES189, was presumed to be responsible for the biodegradation of phthalate.
These results provide insights into the genetic basis of DBP biodegradation in this strain.
American Psychological Association (APA)
Feng, Liang& Liu, Hui& Cheng, Dandan& Mao, Xumei& Wang, Yan& Wu, Zhen…[et al.]. 2018. Characterization and Genome Analysis of a Phthalate Esters-Degrading Strain Sphingobium yanoikuyae SHJ. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126221
Modern Language Association (MLA)
Feng, Liang…[et al.]. Characterization and Genome Analysis of a Phthalate Esters-Degrading Strain Sphingobium yanoikuyae SHJ. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1126221
American Medical Association (AMA)
Feng, Liang& Liu, Hui& Cheng, Dandan& Mao, Xumei& Wang, Yan& Wu, Zhen…[et al.]. Characterization and Genome Analysis of a Phthalate Esters-Degrading Strain Sphingobium yanoikuyae SHJ. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126221