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Distinct Epitopes on CD13 Mediate Opposite Consequences for Cell Adhesion
Joint Authors
Ortega, Enrique
Garay-Canales, Claudia A.
Licona-Limón, Ileana
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-29
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
CD13 is a membrane glycoprotein with aminopeptidase activity, expressed on several cell types, including myeloid cells (dendritic cells, monocytes, macrophages, neutrophils, etc.).
CD13 participates in several functions such as proteolytic regulation of bioactive peptides, viral receptor, angiogenesis, and tumor metastasis.
CD13 has also been proposed to participate in cell adhesion, as crosslinking of CD13 by certain CD13-specific antibodies induces homotypic aggregation of monocytes and heterotypic adhesion of monocytes to endothelial cells.
We generated two monoclonal antibodies (mAbs C and E) that block homotypic aggregation of U-937 monocytic cells induced by CD13-specific mAb 452.
Moreover, the mAbs cause detachment of cells whose aggregation was induced by CD13 crosslinking.
Both mAbs also inhibit heterotypic adhesion of U-937 monocytes to endothelial cells.
mAbs C and E recognize membrane CD13 but bind to epitopes different from that recognized by mAb 452.
Crosslinking of CD13 by mAb C or E is required to inhibit adhesion, as monovalent Fab fragments are not sufficient.
Thus, C and E antibodies recognize a distinct epitope on CD13, and binding to this epitope interferes with both CD13-mediated cell adhesion and enzymatic activity.
These antibodies may represent important tools to study cell-cell interactions mediated by CD13 in physiological and pathological conditions.
American Psychological Association (APA)
Garay-Canales, Claudia A.& Licona-Limón, Ileana& Ortega, Enrique. 2018. Distinct Epitopes on CD13 Mediate Opposite Consequences for Cell Adhesion. BioMed Research International،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1126363
Modern Language Association (MLA)
Garay-Canales, Claudia A.…[et al.]. Distinct Epitopes on CD13 Mediate Opposite Consequences for Cell Adhesion. BioMed Research International No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1126363
American Medical Association (AMA)
Garay-Canales, Claudia A.& Licona-Limón, Ileana& Ortega, Enrique. Distinct Epitopes on CD13 Mediate Opposite Consequences for Cell Adhesion. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1126363
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126363