HSP90: A Novel Target Gene of miRNA-628-3p in A549 Cells

Abstract EN

Lung cancer is one of the leading causes of cancer-related death in the world.

MicroRNA- (miR-) 628-3p plays critical roles in many cancers, including lung cancer.

We investigated how miR-628-3p affected migration and apoptosis in A549 cells.

We used bioinformatics algorithms to predict the miR-628-3p target gene to study the molecular mechanism by which miR-628-3p contributes to lung cancer.

Then, we used the luciferase reporter assay to identify whether heat shock protein 90a (HSP90) is a direct target of miR-628-3p.

Western blotting and quantitative real-time PCR showed that miR-628-3p downregulated HSP90a protein expression via a posttranscriptional mechanism.

We confirm that miR-628-3p promotes apoptosis and inhibits migration in A549 cells by negatively regulating HSP90.

Our results may reveal a novel strategy for lung cancer treatment.