![](/images/graphics-bg.png)
Propofol Reversed Hypoxia-Induced Docetaxel Resistance in Prostate Cancer Cells by Preventing Epithelial–Mesenchymal Transition by Inhibiting Hypoxia-Inducible Factor 1α
Joint Authors
Qian, Jiang
Shen, Sheliang
Chen, Wei
Chen, Nianping
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Prostate cancer is the second most frequently diagnosed cancer worldwide.
Hypoxia-induced epithelial–mesenchymal transition (EMT), driven by hypoxia-inducible factor 1α (HIF-1α), is involved in cancer progression and metastasis.
The present study was designed to explore the role of propofol in hypoxia-induced resistance of prostate cancer cells to docetaxel.
We used the Cell Counting Kit-8 and 5-ethynyl-2′-deoxyuridine incorporation assay to measure cell viability and cell proliferation, respectively, in prostate cancer cell lines.
Then, we detected HIF-1α, E-cadherin, and vimentin expression using western blotting.
Propofol reversed the hypoxia-induced docetaxel resistance in the prostate cancer cell lines.
Propofol not only decreased hypoxia-induced HIF-1α expression, but also reversed hypoxia-induced EMT by suppressing HIF-1α.
Furthermore, small interfering RNA–mediated silencing of HIF-1α reversed the hypoxia-induced docetaxel resistance, although there was little change in docetaxel sensitivity between the hypoxia group and propofol group.
The induction of hypoxia did not affect E-cadherin and vimentin expression, and under the siRNA knockdown conditions, the effects of propofol were obviated.
These data support a role for propofol in regulating EMT in prostate cancer cells.
Taken together, our findings demonstrate that propofol plays an important role in hypoxia-induced docetaxel sensitivity and EMT in prostate cancer cells and that it is a potential drug for overcoming drug resistance in prostate cancer cells via HIF-1α suppression.
American Psychological Association (APA)
Qian, Jiang& Shen, Sheliang& Chen, Wei& Chen, Nianping. 2018. Propofol Reversed Hypoxia-Induced Docetaxel Resistance in Prostate Cancer Cells by Preventing Epithelial–Mesenchymal Transition by Inhibiting Hypoxia-Inducible Factor 1α. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126421
Modern Language Association (MLA)
Qian, Jiang…[et al.]. Propofol Reversed Hypoxia-Induced Docetaxel Resistance in Prostate Cancer Cells by Preventing Epithelial–Mesenchymal Transition by Inhibiting Hypoxia-Inducible Factor 1α. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1126421
American Medical Association (AMA)
Qian, Jiang& Shen, Sheliang& Chen, Wei& Chen, Nianping. Propofol Reversed Hypoxia-Induced Docetaxel Resistance in Prostate Cancer Cells by Preventing Epithelial–Mesenchymal Transition by Inhibiting Hypoxia-Inducible Factor 1α. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1126421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126421