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Blood Donors’ Age, Haemoglobin Type, G6PD Status, and Blood Group Impact Storability of CPDA-1 Banked Whole Blood: A Repeated-Measure Cohort Study in Cape Coast, Ghana
Joint Authors
Adu, Patrick
Ofori, Eric Gyamerah
Kubi, Gilbert Appiah
Kumi, Amos
Gbedoho, Raphael E. K.
Kwakye, Festus Ansah
Sarpong, Emmanuel
Drai, Constantine
Dompreh, Samuel
Sersah, Fredrick Afful
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
The high prevalence of haemoglobin variants and glucose 6-phosphate dehydrogenase disorder (G6PDd) in sub-Saharan Africa means that substantial proportions of donor blood units carry these red cell abnormalities.
Aim.
This study investigated the impact that inherited haemoglobin variants and/or G6PD status have on whole blood banked at 4–6°C for 35 days.
Method.
This repeated-measure cohort study was undertaken on 103 donor blood units collected into blood bag containing CPDA-1 anticoagulant.
On days 0, 7, 14, 21, and 35, full blood count, osmotic-induced haemolysis, and plasma K+ levels were estimated.
Also, on day 0, G6PD status, haemoglobin variants, % foetal haemoglobin, and blood group of donor units were determined using methaemoglobin reductase, cellulose acetate electrophoresis, modified Bekte alkali denaturation assay, and slide haemagglutination test, respectively.
Result.
Overall, although plasma K+ levels increased during storage, donor units from individuals ≥20 years, G6PD normal, Hb AC, or blood group B had comparatively higher percentage change in plasma K+ during storage.
Osmotically induced haemolysis of donor units was significantly decreased in Hb AC (compared with Hb A or AS) donor units on days 7, 14, 21, and 35 (p<0.0001 in each case).
G6PDd donor units had comparatively reduced osmotic-induced lysis compared with G6PD normal units, reaching a statistical significance on day 35 (p=0.043).
Also, Hb AC units had comparatively nonstatistically higher plasma K+ at all time points (compared with Hb A or AS).
Furthermore, whereas donor units from individuals ≥20 years showed significantly higher median free haemoglobin on day 21 (compared to donor <20 years), when donor units were stratified per Hb variants, only Hb AS units had median free haemoglobin below the 0.8% threshold after 35 days’ storage.
Conclusion.
Age of donor, blood group, Hb AC variant, and G6PD status may be important considerations in the storability of whole blood.
American Psychological Association (APA)
Adu, Patrick& Kubi, Gilbert Appiah& Kumi, Amos& Gbedoho, Raphael E. K.& Kwakye, Festus Ansah& Sarpong, Emmanuel…[et al.]. 2020. Blood Donors’ Age, Haemoglobin Type, G6PD Status, and Blood Group Impact Storability of CPDA-1 Banked Whole Blood: A Repeated-Measure Cohort Study in Cape Coast, Ghana. Advances in Hematology،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1126431
Modern Language Association (MLA)
Adu, Patrick…[et al.]. Blood Donors’ Age, Haemoglobin Type, G6PD Status, and Blood Group Impact Storability of CPDA-1 Banked Whole Blood: A Repeated-Measure Cohort Study in Cape Coast, Ghana. Advances in Hematology No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1126431
American Medical Association (AMA)
Adu, Patrick& Kubi, Gilbert Appiah& Kumi, Amos& Gbedoho, Raphael E. K.& Kwakye, Festus Ansah& Sarpong, Emmanuel…[et al.]. Blood Donors’ Age, Haemoglobin Type, G6PD Status, and Blood Group Impact Storability of CPDA-1 Banked Whole Blood: A Repeated-Measure Cohort Study in Cape Coast, Ghana. Advances in Hematology. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1126431
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126431