![](/images/graphics-bg.png)
Optimization of Fluorescent Labeling for In Vivo Nanoimaging of Sarcomeres in the Mouse Heart
Joint Authors
Oyama, Kotaro
Shimozawa, Togo
Kushida, Yasuharu
Terui, Takako
Kobirumaki-Shimozawa, Fuyu
Fukuda, Norio
Ishiwata, Shin'ichi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The present study was conducted to systematically investigate the optimal viral titer as well as the volume of the adenovirus vector (ADV) that expresses α-actinin-AcGFP in the Z-disks of myocytes in the left ventricle (LV) of mice.
An injection of 10 μL ADV at viral titers of 2 to 4 × 1011 viral particles per mL (VP/mL) into the LV epicardial surface consistently expressed α-actinin-AcGFP in myocytes in vivo, with the fraction of AcGFP-expressing myocytes at ~10%.
Our analysis revealed that SL was ~1.90-2.15 μm upon heart arrest via deep anesthesia.
Likewise, we developed a novel fluorescence labeling method of the T-tubular system by treating the LV surface with CellMask Orange (CellMask).
We found that the T-tubular distance was ~2.10-2.25 μm, similar to SL, in the healthy heart in vivo.
Therefore, the present high-precision visualization method for the Z-disks or the T-tubules is beneficial to unveiling the mechanisms of myocyte contraction in health and disease in vivo.
American Psychological Association (APA)
Kobirumaki-Shimozawa, Fuyu& Shimozawa, Togo& Oyama, Kotaro& Kushida, Yasuharu& Terui, Takako& Ishiwata, Shin'ichi…[et al.]. 2018. Optimization of Fluorescent Labeling for In Vivo Nanoimaging of Sarcomeres in the Mouse Heart. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126568
Modern Language Association (MLA)
Kobirumaki-Shimozawa, Fuyu…[et al.]. Optimization of Fluorescent Labeling for In Vivo Nanoimaging of Sarcomeres in the Mouse Heart. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1126568
American Medical Association (AMA)
Kobirumaki-Shimozawa, Fuyu& Shimozawa, Togo& Oyama, Kotaro& Kushida, Yasuharu& Terui, Takako& Ishiwata, Shin'ichi…[et al.]. Optimization of Fluorescent Labeling for In Vivo Nanoimaging of Sarcomeres in the Mouse Heart. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126568
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126568