Quantification of Lappaconitine in Mouse Blood by UPLC-MSMS and Its Application to a Pharmacokinetic Study
Joint Authors
Wen, Congcong
Chen, Fan
Shen, Xiuwei
Huang, Peng
Fu, Huiyan
Jin, Yue
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-06
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Lappaconitine is extracted from Aconitum sinomontanum Nakai, which belongs to the Ranunculaceae.
Lappaconitine is as a diterpenoid alkaloid used as a nonaddictive analgesic.
To assure the rational use of the drug, ultrahigh-pressure liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was conducted to determine lappaconitine in mouse blood and its application to pharmacokinetics.
In this study, khasianine was used as internet standard (IS).
A UPLC BEH C18 column was used for chromatographic separation and the mobile phase consisted of acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid).
The flow rate of was 0.4 mL/min.
Quantitative detection was performed in a multiple reaction monitoring (MRM) mode using an electrospray ionization source in positive mode.
Twenty-four mice were randomly divided into four groups, three of which received 2, 4, and 8 mg/kg lappaconitine by intragastric administration, while the other group received 1 mg/kg lappaconitine by intravenous administration.
After 0.0833, 0.5, 1, 1.5, 2, 3, 4, and 8 h, blood samples were collected and acetonitrile was used for protein precipitation.
A linear calibration relationship (R2 = 0.9979) in the range of 0.1-500 ng/mL in mouse blood indicated good results.
The lower limit of quantitation was 0.1 ng/mL and the limit of detection was 0.04 ng/mL.
The intra-day and inter-day precision were below 13% and 14%, respectively.
The accuracy was 90.1-107.2%, and the recovery exceeded 81.1%.
The matrix effect ranged between 102.1 and 108.8%.
The absolute bioavailability of lappaconitine was 2.0%.
UPLC-MS/MS achieved high sensitivity, speed, and selectivity.
Methodological verification indicated this method as suitable for determination of lappaconitine in mouse blood.
American Psychological Association (APA)
Chen, Fan& Shen, Xiuwei& Huang, Peng& Fu, Huiyan& Jin, Yue& Wen, Congcong. 2019. Quantification of Lappaconitine in Mouse Blood by UPLC-MSMS and Its Application to a Pharmacokinetic Study. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126581
Modern Language Association (MLA)
Chen, Fan…[et al.]. Quantification of Lappaconitine in Mouse Blood by UPLC-MSMS and Its Application to a Pharmacokinetic Study. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1126581
American Medical Association (AMA)
Chen, Fan& Shen, Xiuwei& Huang, Peng& Fu, Huiyan& Jin, Yue& Wen, Congcong. Quantification of Lappaconitine in Mouse Blood by UPLC-MSMS and Its Application to a Pharmacokinetic Study. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1126581
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126581