![](/images/graphics-bg.png)
Quercetin Inhibits Inflammatory Response Induced by LPS from Porphyromonas gingivalis in Human Gingival Fibroblasts via Suppressing NF-κB Signaling Pathway
Joint Authors
Zhang, Tian-liang
Wang, Xia
Cheng, Min
Xiong, Gang
Ji, Wansheng
Wang, Fei
Zhang, Fengxiang
Xue, Peng
Sun, Yanshun
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Quercetin, a natural flavonol existing in many food resources, has been reported to be an effective antimicrobial and anti-inflammatory agent for restricting the inflammation in periodontitis.
In this study, we aimed to investigate the anti-inflammatory effects of quercetin on Porphyromonas gingivalis (P.
gingivalis) lipopolysaccharide- (LPS-) stimulated human gingival fibroblasts (HGFs).
HGFs were pretreated with quercetin prior to LPS stimulation.
Cell viability was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
The levels of inflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), along with chemokine interleukin-8 (IL-8), were determined by enzyme-linked immunosorbent assay (ELISA).
The mRNA levels of IL-1β, IL-6, IL-8, TNF-α, IκBα, p65 subunit of nuclear factor-kappa B (NF-κB), peroxisome proliferator-activated receptor-γ (PPAR-γ), liver X receptor α (LXRα), and Toll-like receptor 4 (TLR4) were measured by real-time quantitative PCR (RT-qPCR).
The protein levels of IκBα, p-IκBα, p65, p-p65, PPAR-γ, LXRα, and TLR4 were characterized by Western blotting.
Our results demonstrated that quercetin inhibited the LPS-induced production of IL-1β, IL-6, IL-8, and TNF-α in a dose-dependent manner.
It also suppressed LPS-induced NF-κB activation mediated by TLR4.
Moreover, the anti-inflammatory effects of quercetin were reversed by the PPAR-γ antagonist of GW9662.
In conclusion, these results suggested that quercetin attenuated the production of IL-1β, IL-6, IL-8, and TNF-α in P.
gingivalis LPS-treated HGFs by activating PPAR-γ which subsequently suppressed the activation of NF-κB.
American Psychological Association (APA)
Xiong, Gang& Ji, Wansheng& Wang, Fei& Zhang, Fengxiang& Xue, Peng& Cheng, Min…[et al.]. 2019. Quercetin Inhibits Inflammatory Response Induced by LPS from Porphyromonas gingivalis in Human Gingival Fibroblasts via Suppressing NF-κB Signaling Pathway. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1126601
Modern Language Association (MLA)
Xiong, Gang…[et al.]. Quercetin Inhibits Inflammatory Response Induced by LPS from Porphyromonas gingivalis in Human Gingival Fibroblasts via Suppressing NF-κB Signaling Pathway. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1126601
American Medical Association (AMA)
Xiong, Gang& Ji, Wansheng& Wang, Fei& Zhang, Fengxiang& Xue, Peng& Cheng, Min…[et al.]. Quercetin Inhibits Inflammatory Response Induced by LPS from Porphyromonas gingivalis in Human Gingival Fibroblasts via Suppressing NF-κB Signaling Pathway. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1126601
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126601