Lats12-Mediated Alteration of Hippo Signaling Pathway Regulates the Fate of Bone Marrow-Derived Mesenchymal Stem Cells
Joint Authors
Li, Lang
Dong, Liang
Wang, Yifeng
Zhang, Xiuhong
Yan, Jie
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Bone marrow-derived mesenchymal stem cells (BMSCs) can be used to enhance lung repair in acute respiratory distress syndrome (ARDS); however, the repairing effect is limited by poor homing and retention of BMSCs.
The purpose of this study was to investigate whether Lats1 and Lats2-mediated alteration of Hippo signaling pathway could promote the differentiation, proliferation, and migration of BMSCs.
BMSCs were transduced by lentiviral vectors for high and low expression of Lats1 and Lats2.
The expression levels of Lats1, Lats2, YAP, and 14-3-3, respectively, were assessed to clarify the regulatory effects of Lats1 and Lats2 on Hippo signaling.
Osteogenic (Runx2 and OSX) and adipogenic (C/EBPα and PPAR-γ) transcription factors were determined to clarify the effects of Hippo signaling on BMSCs differentiation.
The effects of Hippo signaling on BMSCs proliferation and horizontal and vertical migration were also measured by CCK-8, scratch assay, and Transwell migration assay, respectively.
Lentiviral transduction efficiency could reach 93.11%–97.14%.
High and low expression of Lats1 and Lats2 could activate and inhibit the Hippo signaling pathway, respectively.
High and low expression of Lats1 and Lats2 could inhibit and promote BMSCs differentiation into osteoblasts and adipocytes.
High and low expression of Lats1 and Lats2 could inhibit and promote BMSCs proliferation and horizontal and vertical migration, respectively.
Our studies suggest that Lats1/2-meidiated inhibition of Hippo signaling in BMSCs may optimize their effects of tissue repair in ARDS, suggesting a novel strategy for enhancing disease therapeutics.
American Psychological Association (APA)
Li, Lang& Dong, Liang& Wang, Yifeng& Zhang, Xiuhong& Yan, Jie. 2018. Lats12-Mediated Alteration of Hippo Signaling Pathway Regulates the Fate of Bone Marrow-Derived Mesenchymal Stem Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1126621
Modern Language Association (MLA)
Li, Lang…[et al.]. Lats12-Mediated Alteration of Hippo Signaling Pathway Regulates the Fate of Bone Marrow-Derived Mesenchymal Stem Cells. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1126621
American Medical Association (AMA)
Li, Lang& Dong, Liang& Wang, Yifeng& Zhang, Xiuhong& Yan, Jie. Lats12-Mediated Alteration of Hippo Signaling Pathway Regulates the Fate of Bone Marrow-Derived Mesenchymal Stem Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1126621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126621