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Isoleucine Regulates the Synthesis of Pancreatic Enzymes via the Activation of mRNA Expression and Phosphorylation in the Mammalian Target of Rapamycin Signalling Pathways in Pancreatic Tissues
Joint Authors
Cao, Yangchun
Liu, Kai
Liu, Shimin
Guo, Long
Cai, Chuanjiang
Yao, Junhu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study aimed to investigate the effects of isoleucine (Ile) on the synthesis and secretion of digestive enzymes and cellular signalling in the pancreatic tissue of dairy goats.
The pancreatic tissues were incubated in buffer containing 0, 0.40, 0.80, and 1.60 mM Ile.
High levels of Ile significantly increased the buffer release and total concentration of ɑ-amylase in the tissues (P < 0.001).
The total trypsin and chymotrypsin concentrations in each of the Ile groups were significantly higher than those in the control group (P < 0.05); however, lipase was not affected.
High levels of Ile significantly increased ɑ-amylase mRNA expression (P < 0.001) but had no effect on the mRNA expression of trypsin, chymotrypsin, or lipase.
Ile did not affect S6K1 phosphorylation levels.
High levels of Ile significantly increased the expression of the γ isoform of 4EBP1 (P < 0.001), which indicated that the phosphorylation of 4EBP1 was significantly increased.
The phosphorylation level of eEF2 gradually decreased with the addition of Ile (P < 0.001).
These results suggested that high doses of Ile can regulate the excretion of enzymes, especially ɑ-amylase, in the pancreatic tissues of dairy goats by modulating mTOR signalling, and this regulation is independent of the mTOR-S6K1 pathway.
American Psychological Association (APA)
Cao, Yangchun& Liu, Kai& Liu, Shimin& Guo, Long& Cai, Chuanjiang& Yao, Junhu. 2019. Isoleucine Regulates the Synthesis of Pancreatic Enzymes via the Activation of mRNA Expression and Phosphorylation in the Mammalian Target of Rapamycin Signalling Pathways in Pancreatic Tissues. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1126635
Modern Language Association (MLA)
Cao, Yangchun…[et al.]. Isoleucine Regulates the Synthesis of Pancreatic Enzymes via the Activation of mRNA Expression and Phosphorylation in the Mammalian Target of Rapamycin Signalling Pathways in Pancreatic Tissues. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1126635
American Medical Association (AMA)
Cao, Yangchun& Liu, Kai& Liu, Shimin& Guo, Long& Cai, Chuanjiang& Yao, Junhu. Isoleucine Regulates the Synthesis of Pancreatic Enzymes via the Activation of mRNA Expression and Phosphorylation in the Mammalian Target of Rapamycin Signalling Pathways in Pancreatic Tissues. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1126635
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126635