Molecular Cytogenetics of Pisum sativum L. Grown under Spaceflight-Related Stress
Joint Authors
Yurkevich, Olga Yu.
Samatadze, Tatiana E.
Levinskikh, Margarita A.
Zoshchuk, Svyatoslav A.
Signalova, Olga B.
Surzhikov, Sergei A.
Sychev, Vladimir N.
Amosova, Alexandra V.
Muravenko, Olga V.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The ontogenesis and reproduction of plants cultivated aboard a spacecraft occur inside the unique closed ecological system wherein plants are subjected to serious abiotic stresses.
For the first time, a comparative molecular cytogenetic analysis of Pisum sativum L.
(Fabaceae) grown on board the RS ISS during the Expedition-14 and Expedition-16 and also plants of their succeeding (F1 and F2) generations cultivated on Earth was performed in order to reveal possible structural chromosome changes in the pea genome.
The karyotypes of these plants were studied by multicolour fluorescence in situ hybridization (FISH) with five different repeated DNA sequences (45S rDNA, 5S rDNA, PisTR-B/1, microsatellite motifs (AG)12, and (GAA)9) as probes.
A chromosome aberration was revealed in one F1 plant.
Significant changes in distribution of the examined repeated DNAs in karyotypes of the “space grown” pea plants as well as in F1 and F2 plants cultivated on Earth were not observed if compared with control plants.
Additional oligo-(GAA)9 sites were detected on chromosomes 6 and 7 in karyotypes of F1 and F2 plants.
The detected changes might be related to intraspecific genomic polymorphism or plant cell adaptive responses to spaceflight-related stress factors.
Our findings suggest that, despite gradual total trace contamination of the atmosphere on board the ISS associated with the extension of the space station operating life, exposure to the space environment did not induce serious chromosome reorganizations in genomes of the “space grown” pea plants and generations of these plants cultivated on Earth.
American Psychological Association (APA)
Yurkevich, Olga Yu.& Samatadze, Tatiana E.& Levinskikh, Margarita A.& Zoshchuk, Svyatoslav A.& Signalova, Olga B.& Surzhikov, Sergei A.…[et al.]. 2018. Molecular Cytogenetics of Pisum sativum L. Grown under Spaceflight-Related Stress. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126735
Modern Language Association (MLA)
Yurkevich, Olga Yu.…[et al.]. Molecular Cytogenetics of Pisum sativum L. Grown under Spaceflight-Related Stress. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1126735
American Medical Association (AMA)
Yurkevich, Olga Yu.& Samatadze, Tatiana E.& Levinskikh, Margarita A.& Zoshchuk, Svyatoslav A.& Signalova, Olga B.& Surzhikov, Sergei A.…[et al.]. Molecular Cytogenetics of Pisum sativum L. Grown under Spaceflight-Related Stress. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126735
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126735