Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human Osteoblasts In Vitro Treated with Bisphosphonates
Joint Authors
Rodella, Luigi Fabrizio
Rezzani, Rita
Nocini, Pier Francesco
Borsani, Elisa
Bonazza, Veronica
Albanese, Massimo
Zotti, Francesca
Buffoli, Barbara
Inchingolo, Francesco
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Bisphosphonates are primary pharmacological agents against osteoclast-mediated bone loss and widely used in the clinical practice for prevention and treatment of a variety of skeletal conditions, such as low bone density and osteogenesis imperfecta, and pathologies, such as osteoporosis, malignancies metastatic to bone, Paget disease of bone, multiple myeloma, and hypercalcemia of malignancy.
However, long-term bisphosphonate treatment is associated with pathologic conditions including osteonecrosis of the jaw, named BRONJ, which impaired bone regeneration process.
Clinical management of BRONJ is controversy and one recent approach is the use of platelet concentrates, such as Concentrated Growth Factors, alone or together with biomaterials or antioxidants molecules, such as resveratrol.
The aim of the present study was to investigate the in vitro effects of Concentrated Growth Factors and/or resveratrol on the proliferation and differentiation of human osteoblasts, treated or not with bisphosphonates.
Human osteoblasts were stimulated for 3 days in complete medium and for 21 days in mineralization medium.
At the end of the experimental period, the in vitro effect on osteoblast proliferation and differentiation was evaluated using different techniques such as MTT, ELISA for the quantification/detection of osteoprotegerin and bone morphogenetic protein-2, immunohistochemistry for sirtuin 1 and collagen type I, and the Alizarin Red S staining for the rate of mineralization.
Results obtained showed that Concentrated Growth Factors and/or resveratrol significantly increased osteoblast proliferation and differentiation and that the cotreatment with Concentrated Growth Factors and resveratrol had a protective role on osteoblasts treated with bisphosphonates.
In conclusion, these data suggest that this approach could be promised in the clinical management of BRONJ.
American Psychological Association (APA)
Borsani, Elisa& Bonazza, Veronica& Buffoli, Barbara& Nocini, Pier Francesco& Albanese, Massimo& Zotti, Francesca…[et al.]. 2018. Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human Osteoblasts In Vitro Treated with Bisphosphonates. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1126769
Modern Language Association (MLA)
Borsani, Elisa…[et al.]. Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human Osteoblasts In Vitro Treated with Bisphosphonates. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1126769
American Medical Association (AMA)
Borsani, Elisa& Bonazza, Veronica& Buffoli, Barbara& Nocini, Pier Francesco& Albanese, Massimo& Zotti, Francesca…[et al.]. Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human Osteoblasts In Vitro Treated with Bisphosphonates. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1126769
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126769