Inhibition of the Nrf2-TrxR Axis Sensitizes the Drug-Resistant Chronic Myelogenous Leukemia Cell Line K562G01 to Imatinib Treatments
Joint Authors
Xu, Lianrong
Zhao, Yan
Pan, Fei
Zhu, Mengxia
Yao, Liqin
Liu, Yan
Feng, Jiangfang
Xiong, Jie
Chen, Xiuhua
Ren, Fanggang
Tan, Yanhong
Wang, Hongwei
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Nuclear factor erythroid 2-related factor 2 (Nrf2) is involved in tumor drug resistance, but its role in imatinib resistance of chronic myeloid leukemia (CML) remains elusive.
We aimed to investigate the effects of Nrf2 on drug sensitivity, thioredoxin reductase (TrxR) expression, reactive oxygen species (ROS) production, and apoptosis induction in imatinib-resistant CML K562/G01 cells and explored their potential mechanisms.
Stable K562/G01 cells with knockdown of Nrf2 were established by infection of siRNA-expressing lentivirus.
The mRNA and protein expression levels of Nrf2 and TrxR were determined by real-time quantitative polymerase chain reaction and western blot, respectively.
ROS generation and apoptosis were assayed by flow cytometry, while drug sensitivity was measured by the Cell Counting Kit-8 assay.
Imatinib-resistant K562/G01 cells had higher levels of Nrf2 expression than the parental K562 cells at both mRNA and protein levels.
Expression levels of Nrf2 and TrxR were positively correlated in K562/G01 cells.
Knockdown of Nrf2 in K562/G01 cells enhanced the intracellular ROS level, suppressed cell proliferation, and increased apoptosis in response to imatinib treatments.
Nrf2 expression contributes to the imatinib resistance of K562/G01 cells and is positively correlated with TrxR expression.
Targeted inhibition of the Nrf2-TrxR axis represents a potential therapeutic approach for imatinib-resistant CML.
American Psychological Association (APA)
Xu, Lianrong& Zhao, Yan& Pan, Fei& Zhu, Mengxia& Yao, Liqin& Liu, Yan…[et al.]. 2019. Inhibition of the Nrf2-TrxR Axis Sensitizes the Drug-Resistant Chronic Myelogenous Leukemia Cell Line K562G01 to Imatinib Treatments. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1126796
Modern Language Association (MLA)
Xu, Lianrong…[et al.]. Inhibition of the Nrf2-TrxR Axis Sensitizes the Drug-Resistant Chronic Myelogenous Leukemia Cell Line K562G01 to Imatinib Treatments. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1126796
American Medical Association (AMA)
Xu, Lianrong& Zhao, Yan& Pan, Fei& Zhu, Mengxia& Yao, Liqin& Liu, Yan…[et al.]. Inhibition of the Nrf2-TrxR Axis Sensitizes the Drug-Resistant Chronic Myelogenous Leukemia Cell Line K562G01 to Imatinib Treatments. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1126796
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126796