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Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition
Joint Authors
Yin, Yujie
Zhang, Qian
Zhao, Qifei
Ding, Guoyuan
Wei, Cong
Chang, Liping
Li, Hongrong
Bei, Hongying
Wang, Hongtao
Liang, Junqing
Jia, Zhenhua
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Endothelial-to-mesenchymal transition (EndMT) is an essential mechanism in myocardial fibrosis (MF).
Tongxinluo (TXL) has been confirmed to protect the endothelium against reperfusion injury after acute myocardial infarction (AMI).
However, whether TXL can inhibit MF after AMI via inhibiting EndMT remained unknown.
This study aims to identify the role of EndMT in MF after AMI as well as the protective effects and underlying mechanisms of TXL on MF.
The AMI model was established in rats by ligating left anterior descending coronary artery.
Then, rats were administered with high- (0.8 g·kg−1·d−1), mid- (0.4 g·kg−1·d−1), and low- (0.2 g·kg−1·d−1) dose Tongxinluo and benazepril for 4 weeks, respectively.
Cardiac function, infarct size, MF, and related indicators of EndMT were measured.
In vitro, human cardiac microvascular endothelial cells (HCMECs) were pretreated with TXL for 4 h and then incubated in hypoxia conditions for 3 days to induce EndMT.
Under this hypoxic condition, neuregulin-1 (NRG-1) siRNA were further applied to silence NRG-1 expression.
Immunofluorescence microscopy was used to assess expression of endothelial marker of vWF and fibrotic marker of Vimentin.
Related factors of EndMT were determined by Western blot analysis.
TXL treatment significantly improved cardiac function, ameliorated MF, reduced collagen of fibrosis area (types I and III collagen) and limited excessive extracellular matrix deposition (mmp2 and mmp9).
In addition, TXL inhibited EndMT in cardiac tissue and hypoxia-induced HCMECs.
In hypoxia-induced HCMECs, TXL increased the expression of endothelial markers, whereas decreasing the expression of fibrotic markers, partially through enhanced expressions of NRG-1, phosphorylation of ErbB2, ErbB4, AKT, and downregulated expressions of hypoxia inducible factor-1a and transcription factor snail.
After NRG-1 knockdown, the protective effect of TXL on HCMEC was partially abolished.
In conclusion, TXL attenuates MF after AMI by inhibiting EndMT and through activating the NRG-1/ErbB- PI3K/AKT signalling cascade.
American Psychological Association (APA)
Yin, Yujie& Zhang, Qian& Zhao, Qifei& Ding, Guoyuan& Wei, Cong& Chang, Liping…[et al.]. 2019. Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition. BioMed Research International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1126861
Modern Language Association (MLA)
Yin, Yujie…[et al.]. Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition. BioMed Research International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1126861
American Medical Association (AMA)
Yin, Yujie& Zhang, Qian& Zhao, Qifei& Ding, Guoyuan& Wei, Cong& Chang, Liping…[et al.]. Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1126861
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126861