TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway
Joint Authors
Hou, Nengyi
He, Xuelai
Yang, Yuhui
Fu, Junwen
Zhang, Wei
Guo, Zhiyi
Hu, Yang
Liang, Liqin
Xie, Wei
Xiong, Haibo
Wang, Kang
Pang, Minghui
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background/Aims.
TRPV1 is a nonselective Ca2+ channel which has recently been observed in many cancers, while its effect on cell proliferation, apoptosis, metabolism, and cancer development in colorectal cancer (CRC) is still unclear.
In this study, we hypothesized that TRPV1 is a tumor suppressor in CRC development as well as the underlying mechanism.
Methods.
Immunohistochemistry assay was applied to detect the expression of TRPV1 protein in CRC tissues.
HCT116 cell proliferation and apoptosis were measured by CCK-8 and flow cytometry, respectively.
Cellular Ca2+ concentration was measured by Fluo-4/AM-based flow cytometer.
Apoptosis-related proteins were measured by Western blotting.
Results.
In this study, we found that TRPV1 expression was significantly decreased in CRC tissues, compared with CRC-adjacent tissues and normal tissues, respectively.
Then, we found that the TRVP1 agonist capsaicin treatment inhibited CRC growth and induced apoptosis by activating P53.
Subsequent mechanistic study revealed that the TRPV1 induced cytosolic Ca2+ influx to regulate cell apoptosis and p53 activation through calcineurin.
Conclusions.
This study suggests that TRPV1 served as a tumor suppressor in CRC and contributed to the development of novel therapy of CRC.
American Psychological Association (APA)
Hou, Nengyi& He, Xuelai& Yang, Yuhui& Fu, Junwen& Zhang, Wei& Guo, Zhiyi…[et al.]. 2019. TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1126880
Modern Language Association (MLA)
Hou, Nengyi…[et al.]. TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1126880
American Medical Association (AMA)
Hou, Nengyi& He, Xuelai& Yang, Yuhui& Fu, Junwen& Zhang, Wei& Guo, Zhiyi…[et al.]. TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1126880
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126880