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Identification of Key Genes and miRNAs in Osteosarcoma Patients with Chemoresistance by Bioinformatics Analysis
Joint Authors
Xie, Binbin
Li, Yiran
Zhao, Rongjie
Xu, Yuzi
Wu, Yuhui
Wang, Ji
Xia, Dongdong
Han, Weidong
Chen, Dake
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Chemoresistance is a significant factor associated with poor outcomes of osteosarcoma patients.
The present study aims to identify Chemoresistance-regulated gene signatures and microRNAs (miRNAs) in Gene Expression Omnibus (GEO) database.
The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) included positive regulation of transcription, DNA-templated, tryptophan metabolism, and the like.
Then differentially expressed genes (DEGs) were uploaded to Search Tool for the Retrieval of Interacting Genes (STRING) to construct protein-protein interaction (PPI) networks, and 9 hub genes were screened, such as fucosyltransferase 3 (Lewis blood group) (FUT3) whose expression in chemoresistant samples was high, but with a better prognosis in osteosarcoma patients.
Furthermore, the connection between DEGs and differentially expressed miRNAs (DEMs) was explored.
GEO2R was utilized to screen out DEGs and DEMs.
A total of 668 DEGs and 5 DEMs were extracted from GSE7437 and GSE30934 differentiating samples of poor and good chemotherapy reaction patients.
The Database for Annotation, Visualization, and Integrated Discovery (DAVID) was used to perform GO and KEGG pathway enrichment analysis to identify potential pathways and functional annotations linked with osteosarcoma chemoresistance.
The present study may provide a deeper understanding about regulatory genes of osteosarcoma chemoresistance and identify potential therapeutic targets for osteosarcoma.
American Psychological Association (APA)
Xie, Binbin& Li, Yiran& Zhao, Rongjie& Xu, Yuzi& Wu, Yuhui& Wang, Ji…[et al.]. 2018. Identification of Key Genes and miRNAs in Osteosarcoma Patients with Chemoresistance by Bioinformatics Analysis. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126886
Modern Language Association (MLA)
Xie, Binbin…[et al.]. Identification of Key Genes and miRNAs in Osteosarcoma Patients with Chemoresistance by Bioinformatics Analysis. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1126886
American Medical Association (AMA)
Xie, Binbin& Li, Yiran& Zhao, Rongjie& Xu, Yuzi& Wu, Yuhui& Wang, Ji…[et al.]. Identification of Key Genes and miRNAs in Osteosarcoma Patients with Chemoresistance by Bioinformatics Analysis. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1126886
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126886