The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly
Joint Authors
Ulyanova, Vera
Ilinskaya, Olga
Lisevich, Irina
Dudkina, Elena
Zakharchenko, Nataliya
Kusova, Alexandra
Faizullin, Dzhigangir
Zuev, Yuriy
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Supported by crystallography studies, secreted ribonuclease of Bacillus pumilus (binase) has long been considered to be monomeric in form.
Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric.
To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase.
The immutability of binase secondary structure upon transition from low to high protein concentration was registered, suggesting the binase dimerization immediately after translocation through the cell membrane and leading to detection of binase dimers only in the culture fluid regardless of ribonuclease concentration.
Our results made it necessary to take a fresh look at the binase stability and cytotoxicity towards virus-infected or tumor cells.
American Psychological Association (APA)
Ilinskaya, Olga& Ulyanova, Vera& Lisevich, Irina& Dudkina, Elena& Zakharchenko, Nataliya& Kusova, Alexandra…[et al.]. 2018. The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly. BioMed Research International،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1126940
Modern Language Association (MLA)
Ilinskaya, Olga…[et al.]. The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly. BioMed Research International No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1126940
American Medical Association (AMA)
Ilinskaya, Olga& Ulyanova, Vera& Lisevich, Irina& Dudkina, Elena& Zakharchenko, Nataliya& Kusova, Alexandra…[et al.]. The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1126940
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126940