Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice
Joint Authors
Li, Yin
Yuan, Fang
Ma, Jian
Xiang, Xinxin
Lan, He
Xu, Yanhui
Zhao, Jing
Zhang, Weizhen
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
Adipose tissue inflammation is the key linking obesity to insulin resistance.
Over 50% of the interstitial cells in adipose tissue are macrophages, which produce inflammatory cytokines and therefore play an important role in the progression of insulin resistance.
Within this classification view, macrophage biology is driven by two polarization phenotypes, M1 (proinflammatory) and M2 (anti-inflammatory).
The unique functional receptor of ghrelin, growth hormone secretagogue receptor (GHSR), is a classic seven-transmembrane G protein-coupled receptor that is linked to multiple intracellular signaling pathways.
Knockout of GHSR improves the obesity and glucose metabolic disorders, suggesting a crucial role of ghrelin activity in insulin resistance.
Here, we discussed whether macrophage polarization phenotypes in adipose tissue were changed in GHSR knockout (GHSR-/-) mice.
Methods.
GHSR-/- mice were fed with normal chow diet (NCD) or high fat diet (HFD).
Markers of different macrophage polarization phenotypes were detected by real-time RT-PCR.
Results.
The size of adipocytes decreased and interstitial cells, especially infiltrated macrophages, reduced in epididymal adipose tissue of GHSR-/- mice fed with HFD.
Compared with wild type mice, the mRNA levels of inflammatory adipokines such as resistin, IL-6, and PAI-1 were significantly lower in epididymal adipose tissue of GHSR-/- mice, whereas anti-inflammatory adipokine, adiponectin, was significantly higher.
M1 markers, MCP-1, TNF-α, and iNOS, were significantly lower in epididymal adipose tissue of GHSR-/- mice, whereas M2 markers, Arg-1, Mgl-1, were Mrc1, were significantly higher.
Conclusion.
The GHSR-/- mice fed with HFD showed suppressed adipose inflammation, reduced macrophage infiltration, and enhanced M2 polarization of macrophages in adipose tissue, which improved insulin sensitivity.
American Psychological Association (APA)
Yuan, Fang& Ma, Jian& Xiang, Xinxin& Lan, He& Xu, Yanhui& Zhao, Jing…[et al.]. 2018. Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126989
Modern Language Association (MLA)
Yuan, Fang…[et al.]. Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1126989
American Medical Association (AMA)
Yuan, Fang& Ma, Jian& Xiang, Xinxin& Lan, He& Xu, Yanhui& Zhao, Jing…[et al.]. Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1126989
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126989