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FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens
Joint Authors
Wang, Yan
Li, Diyan
Han, Shunshun
Cui, Can
Yin, Huadong
Luo, Bin
Shen, Xiaoxu
Yang, Fuling
Liu, Zihao
Zhu, Qing
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
FOXO3, which encodes the transcription factor forkhead box O-3 (FoxO3), is a member of the FOXO subfamily of the forkhead box (FOX) family.
FOXO3 can be negatively regulated by its phosphorylation by the PI3K/Akt signaling pathway and ultimately drives apoptosis when activated.
In mammalian ovaries, the FOXO3 protein regulates atresia and follicle growth by promoting apoptosis of ovarian granulosa cells.
Nonetheless, the specific effects of the FOXO3 protein on granulosa apoptosis of avian ovaries have not been elucidated.
Therefore, we studied FOXO3 expression in follicles with different organization and at all hierarchical levels of chicken follicles.
Via an immunofluorescence assay, the chicken follicular theca at all hierarchical levels were found to be strongly stained with an anti-FOXO3 antibody.
In chicken primary ovarian granulosa cells, mRNA levels of proapoptotic factors BNIP3 and BCL2L11 decreased in the absence of FOXO3, and so did PARP-1 and cleaved caspase 3 protein levels.
After treatment with a recombinant FOXO3 protein, PARP-1 and caspase 3 protein levels increased, along with mRNA levels of Bnip3 and BCL2L11 (significantly, p<0.05).
In addition, FOXO3 was downregulated in chicken granulosa cells when different estradiol or FSH concentrations were applied.
In conclusion, FOXO3 is expressed in chicken reproductive tissues, including follicles and ovarian granulosa cells, and promotes apoptosis of chicken ovarian granulosa cells.
American Psychological Association (APA)
Cui, Can& Han, Shunshun& Yin, Huadong& Luo, Bin& Shen, Xiaoxu& Yang, Fuling…[et al.]. 2019. FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1126998
Modern Language Association (MLA)
Cui, Can…[et al.]. FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1126998
American Medical Association (AMA)
Cui, Can& Han, Shunshun& Yin, Huadong& Luo, Bin& Shen, Xiaoxu& Yang, Fuling…[et al.]. FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1126998
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1126998