Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay
Joint Authors
Ma, Lei
Zeng, Fanwen
Huang, Bihong
Cong, Feng
Huang, Ren
Ma, Jingyun
Guo, Pengju
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae.
It causes watery diarrhea, vomiting, and dehydration in newborn piglets.
A sensitive RT-PCR method is urgently required to detect PDCoV infection.
In this study, we developed and evaluated a conventional RT-PCR assay and a SYBR green-based real-time RT-PCR assay that targeted the PDCoV n gene.
Both assays are specific and have the same limit of detection at 2 × 101 copies of RNA molecules per reaction.
Eighty-four clinical samples were subjected to both conventional RT-PCR and real-time RT-PCR, and the same positive rate (41.7%) was achieved, which was much higher than the positive rate (26.2%) using a previously described one-step RT-PCR technique.
In summary, a conventional RT-PCR technique was successfully established for the detection of PDCoV with the same detection limit as a SYBR green-based real-time RT-PCR assay.
American Psychological Association (APA)
Ma, Lei& Zeng, Fanwen& Huang, Bihong& Cong, Feng& Huang, Ren& Ma, Jingyun…[et al.]. 2018. Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay. BioMed Research International،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1127040
Modern Language Association (MLA)
Ma, Lei…[et al.]. Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay. BioMed Research International No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1127040
American Medical Association (AMA)
Ma, Lei& Zeng, Fanwen& Huang, Bihong& Cong, Feng& Huang, Ren& Ma, Jingyun…[et al.]. Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1127040
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127040