Effect of Lead on Human Middle Ear Epithelial Cells
Joint Authors
Kim, Shin Hye
Shin, Sun Hwa
Go, Yoon Young
Chae, Sung-Won
Song, Jae-Jun
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Lead is a ubiquitous metal in the environment, but no studies have examined lead toxicity on the middle ear.
Here, we investigated lead toxicity and its mechanism in human middle ear epithelial cells (HMEECs).
Moreover, we investigated the protective effects of amniotic membrane extract (AME) and chorionic membrane extract (CME) against lead toxicity in HMEECs.
Cell viability was analyzed using the cell counting kit, and reactive oxygen species (ROS) activity was measured using a cellular ROS detection kit.
After lead(II) acetate trihydrate treatment, mRNA levels of various genes were assessed by semiquantitative real-time polymerase chain reaction.
Following treatment with AME or CME after lead exposure, the changes in cell viability, ROS activity, and gene expression were analyzed.
Exposure to >100 μg/mL of lead(II) acetate trihydrate caused a significant decrease in cell viability and increased ROS production in HMEECs.
Lead exposure significantly increased the mRNA expression of genes encoding inflammatory cytokines and mucins.
Administration of AME or CME restored cell viability, reduced ROS activity, and ameliorated mRNA levels.
Our findings suggest that environmental lead exposure is related to the development of otitis media, and AME and CME may have antioxidative and anti-inflammatory effects against lead toxicity.
American Psychological Association (APA)
Kim, Shin Hye& Shin, Sun Hwa& Go, Yoon Young& Chae, Sung-Won& Song, Jae-Jun. 2018. Effect of Lead on Human Middle Ear Epithelial Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127064
Modern Language Association (MLA)
Kim, Shin Hye…[et al.]. Effect of Lead on Human Middle Ear Epithelial Cells. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1127064
American Medical Association (AMA)
Kim, Shin Hye& Shin, Sun Hwa& Go, Yoon Young& Chae, Sung-Won& Song, Jae-Jun. Effect of Lead on Human Middle Ear Epithelial Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127064
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127064