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Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA
Joint Authors
Yusof, Nor Azah
Mohammad, Faruq
Al balawi, Aisha Nawaf
Kamaruzaman, Sazlinda
Al Abbosh, Khulood Fahad
Wasoh, H.
al-Lohedan, Hamad A.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The present study has synthesized poly(4,4'-cyclohexylidene bisphenol oxalate) by the condensation of oxalyl chloride with 4,4'-cyclohexylidene bisphenol, where its efficacy was tested for the solid-phase extraction of DNA.
The synthesized polymer in the form of a white powder was characterized by FTIR, TGA-DTG, SEM, and BET analysis.
The study utilized solid-phase application of the resulting polymer to extract DNA.
The analysis of results provided the information that the extraction efficiency is a strong dependent of polymer amount and binding buffer type.
Among the three types of buffers tested, the GuHCl buffer produced the most satisfactory results in terms of yield and efficiency of extraction.
Moreover, the absorbance ratio of A260/A280 in all of the samples varied from 1.682 to 1.491, thereby confirming the capability of poly(4,4'-cyclohexylidene bisphenol oxalate) to elute pure DNA.
The results demonstrated an increased DNA binding capacity with respect to increased percentage of the polymer.
The study has concluded that poly(bisphenol Z oxalate) can be applied as one of the potential candidates for the high efficiency extraction of DNA by means of a simple, cost-effective, and environmentally friendly approach compared to the other traditional solid-phase methods.
American Psychological Association (APA)
Al balawi, Aisha Nawaf& Yusof, Nor Azah& Kamaruzaman, Sazlinda& Mohammad, Faruq& Wasoh, H.& Al Abbosh, Khulood Fahad…[et al.]. 2019. Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA. BioMed Research International،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1127086
Modern Language Association (MLA)
Al balawi, Aisha Nawaf…[et al.]. Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA. BioMed Research International No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1127086
American Medical Association (AMA)
Al balawi, Aisha Nawaf& Yusof, Nor Azah& Kamaruzaman, Sazlinda& Mohammad, Faruq& Wasoh, H.& Al Abbosh, Khulood Fahad…[et al.]. Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1127086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127086