miRNA and mRNA Integration Network Construction Reveals Novel Key Regulators in Left-Sided and Right-Sided Colon Adenocarcinoma
Joint Authors
Yu, Xiangyang
Yang, Likun
Li, Lin
Ma, Junhong
Yang, Shimin
Zou, Changlin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
The distinction between right-sided and left-sided colon adenocarcinoma has recently received considerable.
This study aims to identify key MicroRNA (miRNA) and mRNAs in right-sided colon adenocarcinoma (RSCOAD) and left-sided colon adenocarcinoma (LSCOAD) by TCGA integration analysis.
Methods.
The miRNA and mRNA expression profiles of a large group of patients with RSCOAD and LSCOAD were obtained from TCGA.
The differentially expressed miRNAs (DEmiRNAs) and mRNAs (DEmRNAs) were identified by TCGA integration analysis.
The optimal diagnostic miRNA biomarkers for RSCOAD and LSCOAD were identified by Boruta algorithm.
We established classification models to distinguish RSCOAD and LSCOAD.
Protein-protein interaction (PPI) network analysis, DEmiRNA-DEmRNA interaction analysis, and functional annotation were performed.
The expression of selected DEmiRNAs and DEmRNAs was validated by qRT-PCR.
Results.
A total of 2534 DEmRNAs (940 downregulated and 1594 upregulated mRNAs) and 54 DEmiRNAs (22 downregulated and 32 upregulated miRNAs) between RSCOAD and LSCOAD were identified.
The feature selection procedure was to obtain 22 optimal diagnostic miRNAs biomarkers in RSCOAD compared to LSCOAD.
The AUC of the random forests model was 0.869 and the specificity and sensitivity of this model were 79% and 84.6%, respectively.
Three DEmiRNAs (hsa-miR-224-5p, hsa-miR-155-5p, and hsa-miR-31-5p) and five DEmRNAs (CXCR4, SMAD4, KRAS, FITM2, and PLAGL2) were identified key DEmiRNAs and DEmRNAs in RSCOAD compared to LSCOAD.
The qRT-PCR results of CXCR4, FITM2, TFAP2A, ULBP2, hsa-miR-224-5p, and hsa-miR-155-5p were consistent with our integrated analysis.
Conclusion.
A total of three DEmiRNAs (hsa-miR-224-5p, hsa-miR-155-5p, and hsa-miR-31-5p) and five DEmRNAs (CXCR4, SMAD4, KRAS, FITM2, and PLAGL2) may be involved in the pathogenesis of RSCOAD and LSCOAD which may make a contribution for understanding mechanisms and developing therapeutic strategies for RSCOAD and LSCOAD.
American Psychological Association (APA)
Yang, Likun& Li, Lin& Ma, Junhong& Yang, Shimin& Zou, Changlin& Yu, Xiangyang. 2019. miRNA and mRNA Integration Network Construction Reveals Novel Key Regulators in Left-Sided and Right-Sided Colon Adenocarcinoma. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127126
Modern Language Association (MLA)
Yang, Likun…[et al.]. miRNA and mRNA Integration Network Construction Reveals Novel Key Regulators in Left-Sided and Right-Sided Colon Adenocarcinoma. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1127126
American Medical Association (AMA)
Yang, Likun& Li, Lin& Ma, Junhong& Yang, Shimin& Zou, Changlin& Yu, Xiangyang. miRNA and mRNA Integration Network Construction Reveals Novel Key Regulators in Left-Sided and Right-Sided Colon Adenocarcinoma. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127126
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127126