Evaluation of Tyrosinase Inhibitory, Antioxidant, Antimicrobial, and Antiaging Activities of Magnolia officinalis Extracts after Aspergillus niger Fermentation
Joint Authors
Wu, Lichun
Chen, Chihyu
Cheng, Chiuyu
Dai, Hang
Ai, Yazhao
Lin, Chiahui
Chung, Yingchien
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study intended to improve physiological characteristics of Magnolia officinalis bark (MOB) extracts by Aspergillus niger fermentation.
M.
officinalis bark was extracted using distilled water, 95% ethanol, and methanol, and it was then fermented by A.
niger.
The physiological characteristics of the fermented extracts, namely, tyrosinase inhibitory activity, antioxidant activity, antibacterial activity, and anti-skin-aging activity, were evaluated and compared with those of unfermented extracts.
To determine the safety of the fermented extracts, their cytotoxicity was analyzed by measuring the cell viability of CCD-966SK and human epidermal melanocytes (HEMn) after exposure.
The fermented methanol extract exhibited the highest antityrosinase activity, total phenolic content, and antioxidant activity.
The total phenolic content of the extracts fermented by A.
niger was 3.52 times greater than that of the unfermented extracts.
The optimal IC50 values for tyrosinase inhibition and 2,2-diphenyl-1-picrylhydrazyl (DPPH) removal by the A.
niger-fermented extracts were 30 and 12 μg/mL, respectively.
The fermented methanol extracts inhibited skin-aging-related enzymes such as collagenase, elastase, MMP-1, and MMP-2.
Compared with the unfermented extracts, the fermented extracts also contained greater antibacterial activity against tested stains including MRSA.
These results could be attributed to an increase in the concentration of original active compounds and the biosynthesis of new compounds during fermentation.
In cytotoxicity assays, the A.
niger-fermented extracts were nontoxic to CCD-966SK cells, even at 500 μg/mL.
Hence, in general, methanol-extracted M.
officinalis fermented by A.
niger for 72 h has the most active antioxidant, skincare, or antiaging compounds for healthy food or cosmetics applications.
American Psychological Association (APA)
Wu, Lichun& Chen, Chihyu& Cheng, Chiuyu& Dai, Hang& Ai, Yazhao& Lin, Chiahui…[et al.]. 2018. Evaluation of Tyrosinase Inhibitory, Antioxidant, Antimicrobial, and Antiaging Activities of Magnolia officinalis Extracts after Aspergillus niger Fermentation. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127144
Modern Language Association (MLA)
Wu, Lichun…[et al.]. Evaluation of Tyrosinase Inhibitory, Antioxidant, Antimicrobial, and Antiaging Activities of Magnolia officinalis Extracts after Aspergillus niger Fermentation. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1127144
American Medical Association (AMA)
Wu, Lichun& Chen, Chihyu& Cheng, Chiuyu& Dai, Hang& Ai, Yazhao& Lin, Chiahui…[et al.]. Evaluation of Tyrosinase Inhibitory, Antioxidant, Antimicrobial, and Antiaging Activities of Magnolia officinalis Extracts after Aspergillus niger Fermentation. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127144
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127144